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Cell type-dependent modulation of senescence features using Weo electrolyzed water.

Brenda L Court-Vazquez1, Shirley A Arroyo-Vizcarrondo1, Jonathan A Poli1

  • 1Weo LLC, Research and Development Department, Miami, FL 33136, USA.

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Electrolyzed-reduced water (WEW) shows antioxidant properties, reducing cellular senescence in normal cells but increasing stress in cancer cells. This suggests WEW may impact longevity and age-related diseases.

Keywords:
breast cancer cellscellular senescencelung fibroblastsoxidative stresssenescence-associated secretory phenotypesenomorphic

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Area of Science:

  • Cellular Biology
  • Oxidative Stress Research
  • Aging and Longevity Studies

Background:

  • Oxidative stress, an imbalance between reactive oxygen species (ROS) and antioxidant defenses, contributes to cellular senescence, a hallmark of aging.
  • Cellular senescence is implicated in age-related diseases and conditions.
  • Electrolyzed-reduced water (WEW) possesses antioxidant properties that scavenge ROS.

Purpose of the Study:

  • To investigate the potential of WEo electrolyzed water (WEW) to modulate the phenotype of senescent cells.
  • To assess the molecular impact of WEW on senescence markers, senescence-associated secretory phenotype (SASP) factors, and stress response genes in different cell types.

Main Methods:

  • Normal human lung fibroblasts (BJ) and breast cancer cells (T47D) were induced into senescence using hydrogen peroxide (H₂O₂).
  • The effects of WEW treatment on senescence markers (SA-β-gal activity, EdU incorporation, p21 expression) were evaluated.
  • Changes in SASP factors and stress response genes were analyzed in both cell types following WEW treatment.

Main Results:

  • WEW modulated cellular senescence markers similarly in both BJ and T47D cells.
  • WEW exhibited cell type-dependent effects on SASP factors and stress response genes.
  • WEW decreased SASP factors and stress genes in normal BJ cells but increased them in T47D cancer cells.

Conclusions:

  • WEW acts as a protective factor in normal cells by reducing oxidative stress and senescence.
  • WEW sensitizes cancer cells to stress, potentially aiding their elimination.
  • WEW's differential senomorphic effects suggest potential benefits for longevity and age-related diseases.