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Streptomyces phaeochromogenes BV-204, K-1115A Anthraquinone-Producing Strain: A New Protein Biosynthesis Inhibitor
A R Belik1, Yu V Zakalyukina1,2, V A Alferova3
1Sirius University of Science and Technology, Sochi, 354340 Russian Federation.
Acta Naturae
|May 3, 2024
Summary
This study introduces a novel reporter system for antibiotic discovery, identifying K-1115A from S. phaeochromogenes. K-1115A shows promising antibacterial activity and translation inhibition, suggesting potential for new drug development.
Area of Science:
- Microbiology
- Drug Discovery
- Molecular Biology
Background:
- The search for novel antibiotics often leads to rediscovery of known compounds.
- Target-oriented screening using reporter microorganisms offers a solution to identify new antibiotic properties.
- Existing screening methods can be improved for higher sensitivity and mechanism identification.
Purpose of the Study:
- To evaluate the efficacy of the pDualrep2 dual reporter system for antibiotic screening.
- To identify and characterize novel biologically active substances with antibiotic potential.
- To investigate the mechanism of action and therapeutic potential of the identified compound K-1115A.
Main Methods:
- High-throughput screening utilizing the pDualrep2 dual reporter system.
- Isolation and characterization of the producing strain *S. phaeochromogenes* BV-204.
- In vitro confirmation of antibiotic activity and translation inhibition.
- Cytotoxicity assessment against the HEK293 cell line.
Main Results:
- The pDualrep2 system successfully identified K-1115A from *S. phaeochromogenes*.
- K-1115A demonstrated significant antibiotic activity against *S. aureus* (MRSA) and *B. subtilis*.
- K-1115A was confirmed to inhibit bacterial translation in a cell-free system.
- K-1115A exhibited therapeutic indices of 2 and 8 against HEK293 cells.
Conclusions:
- The pDualrep2 reporter system is a sensitive and effective tool for antibiotic discovery.
- K-1115A is a promising antibiotic candidate with a novel mechanism of action.
- K-1115A serves as a foundation for developing semi-synthetic derivatives with enhanced therapeutic properties.
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