Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K
In-situ Hybridization02:31

In-situ Hybridization

9.4K
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
9.4K
CRISPR01:59

CRISPR

50.8K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
50.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In situ imaging of proximal biomolecules via Proximity Anchored Modules Assembly.

Journal of nanobiotechnology·2026
Same author

Synergistic antioxidant and gene supplementation for high-efficacy retinitis pigmentosa therapy.

Science advances·2026
Same author

An autosampling microfluidic chip enables FEN1-aided recombinase polymerase amplification to rapidly detect multiple urinary tract infection pathogens on-site.

Mikrochimica acta·2026
Same author

EVA-YOLOv8: an improved YOLOv8 model integrating multi-scale attention mechanism and vision transformer for multi-class road crack detection.

Scientific reports·2026
Same author

A tailored phosphorothioate coordinator enables CRISPR/Cas in-situ amplification.

Nucleic acids research·2026
Same author

Enhancing mouse fundus imaging with OCT embedding medium: Prolonging imaging duration and improving image quality.

Experimental eye research·2026

Related Experiment Video

Updated: Jun 27, 2025

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

11.7K

Multiple RNA Rapid In Situ Imaging Based on Cas9 Code Key System.

Ruiwei Hu1,2, Wei Yang3, Jia Li1

  • 1The Center for Clinical Molecular Medical Detection, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400042, China.

Small Methods
|May 3, 2024
PubMed
Summary
This summary is machine-generated.

A novel Cas9 code key system simplifies RNA in situ imaging, enabling rapid, efficient multi-mRNA detection in tumor cells. This breakthrough offers enhanced clinical tumor typing and molecular investigation capabilities.

Keywords:
CRISPR/Cas9FFPE samplekey probe encodermultiple RNA in situ imagingtumor typing

More Related Videos

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

11.8K
Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.2K

Related Experiment Videos

Last Updated: Jun 27, 2025

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

11.7K
Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

11.8K
Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.2K

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genomics

Background:

  • Existing RNA in situ imaging methods often involve complex nucleic acid self-assembly, leading to cumbersome procedures and limitations in efficiency.
  • The need for simplified and more efficient techniques for multiplexed RNA analysis in biological samples is critical.

Purpose of the Study:

  • To develop a simplified and highly efficient system for multiplexed RNA in situ imaging.
  • To overcome the limitations of conventional parallel signaling analysis in RNA detection.

Main Methods:

  • Development of a Cas9 code key system utilizing key probe (KP) encoders and CRISPR/Cas9 signal exporters.
  • Integration of the Cas9 code key system with T-strand displacement amplification (T-SDA) for signal amplification.
  • Application of the system for multi-mRNA imaging in tumor cells and clinical pathology slices.

Main Results:

  • The Cas9 code key system enables efficient "many-to-one" tandem signaling with a single sgRNA/Cas9 complex.
  • The system demonstrates non-collateral cleavage activity-dependent automatic signaling output.
  • Rapid multi-mRNA imaging profiles were generated in under an hour with a single step, showing high efficiency and simplified reaction processes.

Conclusions:

  • The developed Cas9 code key system significantly simplifies RNA in situ imaging processes.
  • This approach provides a rapid and efficient method for multiplexed RNA detection, crucial for clinical applications.
  • The system offers reliable technical support for clinical tumor typing and molecular mechanism investigation.