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Updated: May 11, 2026
Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry
Published on: May 21, 2018
Differential antigenicity of individual Mycoplasma hyorhinis variable lipoproteins
Precy D Magtoto1, Bailey L Arruda2, Ronaldo L Magtoto3
1College of Veterinary Medicine, Pampanga State Agricultural University, Pampanga, the Philippines; College of Arts and Sciences, University of the Philippines Los Baños, Laguna, the Philippines.
Abstract:
The Mycoplasma hyorhinis (Mhr) variable lipoprotein (Vlp) family, comprising Vlps A, B, C, D, E, F, and G, are highly variable in expression, size, and cytoadhesion capabilities across Mhr strains. The 'Vlp system' plays a crucial role in cytoadhesion, immune evasion, and in eliciting a host immunologic response. This pilot study described the development of Vlp peptide-based ELISAs to evaluate the antigenic reactivity of individual Vlps against Mhr antisera collected throughout a longitudinal study focused on Mhr strain 38983, reproducing Mhr-associated disease under experimental conditions. Specifically, serum samples were collected at day post-inoculation 0, 7, 10, 14, 17, 21, 24, 28, 35, 42, 49, and 56 from Mhr- and mock (Friis medium)-inoculated cesarean-derived, colostrum-deprived pigs. Significant Mhr-specific IgG responses were detected at specific time points throughout the infection, with some variations for each Vlp. Overall, individual Vlp ELISAs showed consistently high accuracy rates, except for VlpD, which would likely be associated with its expression levels or the anti-Vlp humoral immune response specific to the Mhr strain used in this study. This study provides the basis and tools for a more refined understanding of these Vlp- and Mhr strain-specific variations, which is foundational in understanding the host immune response to Mhr.
Insights
This study developed peptide-based ELISAs to detect immune responses to Mycoplasma hyorhinis (Mhr) variable lipoproteins (Vlps). The assays accurately identified Mhr-specific antibodies, aiding understanding of host immunity to Mhr infections.
Area of Science:
- Veterinary Immunology
- Microbial Pathogenesis
- Molecular Biology
Background:
- Mycoplasma hyorhinis (Mhr) variable lipoproteins (Vlps) are crucial for bacterial cytoadhesion and immune evasion.
- Vlp expression, size, and function vary significantly among Mhr strains.
- Understanding Vlp-specific immune responses is key to controlling Mhr-associated diseases.
Purpose of the Study:
- To develop and validate Vlp peptide-based ELISAs for assessing antigenic reactivity.
- To evaluate humoral immune responses to individual Vlps during experimental Mhr infection in pigs.
- To investigate Mhr strain-specific variations in host immune responses.
Main Methods:
- Development of Vlp peptide-based ELISAs.
- Longitudinal collection of pig serum samples post-Mhr inoculation (0-56 days).
- Detection of Mhr-specific IgG responses using developed ELISAs.
Main Results:
- Significant Mhr-specific IgG responses were detected at various time points post-infection.
- Individual Vlp ELISAs demonstrated high accuracy, with notable exceptions for VlpD.
- Variations in immune response were observed for different Vlps, potentially linked to expression levels or strain specificity.
Conclusions:
- Peptide-based Vlp ELISAs are effective tools for studying Mhr-specific humoral immunity.
- ELISA results highlight Vlp and Mhr strain-specific variations in host immune responses.
- This work provides a foundation for further research into Mhr pathogenesis and host-pathogen interactions.
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