Laboratory sprayer for dsRNA application: Design and bioassay validation

Area of Science:

• Agricultural Science
• Molecular Biology
• Entomology

Background:

• Commercial laboratory spraying equipment for testing preparations is limited, hindering field-comparable results.
• RNA interference (RNAi) offers a sustainable plant protection method with minimal environmental impact.
• Accurate laboratory application of double-stranded RNA (dsRNA) is crucial for developing effective RNAi-based pest control strategies.

Purpose of the Study:

• To develop and evaluate a portable spray device for consistent laboratory application of dsRNA.
• To assess the suitability of the device for dsRNA delivery across different plant species and leaf textures.
• To validate dsRNA detection post-treatment for reliable bioassay outcomes.

Main Methods:

• Construction of a portable spray device designed for laboratory use.
• Conducting bioassays on three distinct plant species with varying leaf surface characteristics.
• Quantifying dsRNA presence in plant tissues at 3 days post-treatment.

Main Results:

• The developed portable spray device demonstrated suitability for dsRNA application in laboratory settings.
• dsRNA was successfully detected in all tested plant species 3 days after treatment.
• The device facilitated consistent and repeatable dsRNA delivery, crucial for bioassays.

Conclusions:

• The novel portable spray device enables reliable laboratory-based dsRNA application for plant protection research.
• This tool supports the development of field-comparable RNAi strategies by ensuring consistent dsRNA delivery.
• The findings pave the way for more efficient and environmentally friendly pest management solutions.