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In Situ Imaging of Proteins Using DNA-PAINT Super-Resolution Microscopy
Florian Schueder1,2, Ralf Jungmann3,4
1Department of Cell Biology, Yale School of Medicine, New Haven, CT, USA.
None:
The spatial resolution of conventional light microscopy is restricted by the diffraction limit to hundreds of nanometers. Super-resolution microscopy enables single digit nanometer resolution by circumventing the diffraction limit of conventional light microscopy. DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) belongs to the family of single-molecule localization super-resolution approaches. Unique features of DNA-PAINT are that it allows for sub-nanometer resolution, spectrally unlimited multiplexing, proximity detection, and quantitative counting of target molecules. Here, we describe prerequisites for efficient DNA-PAINT microscopy.
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