Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Variation in <i>SNX29</i> and Acute Vasodilator Response in Pulmonary Arterial Hypertension.

Circulation research·2026

Same author

Involvement of Non-Muscle Myosin Light Chain Kinase Nitration in Molecular Regulation of Inflammation-Induced Endothelial Cell Barrier Dysfunction.

Cells·2026

Same author

Rapid antithrombin assay from human blood plasma utilizing smartphone-based flow observation on paper chips.

PLOS digital health·2026

Same author

Microfluidic Sensors Integrated with Smartphones for Applications in Forensics, Agriculture, and Environmental Monitoring.

Micromachines·2025

Same author

Fluorescence-based spectrometric and imaging methods and machine learning analyses for microbiota analysis.

Mikrochimica acta·2025

Same author

eNAMPT Is a Novel DAMP and Therapeutic Target in Human and Murine Pulmonary Fibrosis.

American journal of respiratory cell and molecular biology·2025

Same journal

Dual phosphoregulatory mechanisms of condensin I revealed by biochemical reconstitution.

PNAS nexus·2026

Same journal

Vanin-1 deficiency enhances host tolerance to influenza infection by modulating cellular redox status.

PNAS nexus·2026

Same journal

Free will in the eyes of Muslims and Christians.

PNAS nexus·2026

Same journal

Paradoxical coexistence of superconductivity and magnetism, and explaining unexpected preferred domain orientations.

PNAS nexus·2026

Same journal

Large language models instantiate evolutionarily robust strategies of cooperation.

PNAS nexus·2026

Same journal

Compression of functional gradients during early nonrapid eye movement sleep.

PNAS nexus·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 27, 2025

Fluorescence detection methods for microfluidic droplet platforms

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Development of a cloud-based flow rate tool for eNAMPT biomarker detection.

Bailey C Buchanan¹, Yisha Tang¹, Hannah Lopez²

¹Department of Biomedical Engineering, The University of Arizona, 1127 E. James E. Rogers Way, Tucson, AZ 85721, USA.

|May 7, 2024

Summary

This summary is machine-generated.

A rapid point-of-care test using particle immunoagglutination on paper microfluidics can detect extracellular nicotinamide phosphoribosyltransferase (eNAMPT) levels in under a minute. This assay aids in assessing inflammatory disease severity and guiding therapeutic decisions.

Keywords:

Google Colab acute respiratory distress syndrome capillary action paper microfluidics smartphone

More Related Videos

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection

Published on: October 25, 2013

Related Experiment Videos

Last Updated: Jun 27, 2025

Fluorescence detection methods for microfluidic droplet platforms

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection

Published on: October 25, 2013

Area of Science:

Biochemistry
Biomarker Discovery
Point-of-Care Diagnostics

Background:

Extracellular nicotinamide phosphoribosyltransferase (eNAMPT) is a biomarker for inflammatory diseases.
Neutralizing eNAMPT with monoclonal antibodies shows therapeutic potential in preclinical models.
Rapid point-of-care (POC) detection of eNAMPT is needed to assess anti-eNAMPT therapy efficacy.

Purpose of the Study:

To develop and validate a rapid POC assay for quantifying eNAMPT in human samples.
To assess the feasibility of using a paper microfluidic platform for eNAMPT detection.
To enable timely patient stratification and therapeutic guidance.

Main Methods:

Particle immunoagglutination assay on a paper microfluidic platform.
Quantification via flow rate measurement analyzed by smartphone and Google Colab.
Optimization using horizontal flow and immunoagglutination binding models.

Main Results:

Assay detected eNAMPT in whole blood and plasma within 1 minute.
Limit of detection: 1-20 pg/mL (0.1-0.2 ng/mL undiluted).
Linear range: 5-40 pg/mL; distinguished low, mid, and high eNAMPT concentrations.

Conclusions:

A low-cost, rapid POC assay for eNAMPT levels was successfully developed.
The assay enables quick determination of eNAMPT in blood/plasma.
This technology can aid clinical trial patient stratification and therapeutic decision-making for anti-eNAMPT treatments.