Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

6.1K
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
6.1K
Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

3.5K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
3.5K
COP Coated Vesicles00:59

COP Coated Vesicles

7.8K
Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
7.8K
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

6.9K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
6.9K
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

3.1K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
3.1K
Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

11.1K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
11.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tunable self-assembling cellular microarray for single-neutrophil vital and suicidal extracellular traps.

Lab on a chip·2026
Same author

Hemopexin Purification From Human Cohn Fraction IV Paste and Its Biophysical Characterization and Functional Evaluation in Sickle Cell Disease Mice.

Biotechnology and bioengineering·2026
Same author

Metabolic Collapse in Acute CNS Injury: A Spatiotemporal Framework Linking Redox Failure, Ferroptosis, and Neurovascular Dysfunction.

Antioxidants & redox signaling·2026
Same author

Temporal trend of serum vitamin profiles and the association with non-alcoholic fatty liver disease.

Frontiers in nutrition·2026
Same author

Plasma EV Proteomics Identifies ECM Remodeling and Inflammatory Proteins LUM and C7 as Candidate Biomarkers in FSHD.

Annals of clinical and translational neurology·2026
Same author

Multidimensional Cellular Micro-Compartments to Model Invasive Lobular Carcinoma Dormancy.

Advanced healthcare materials·2026
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026
Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Jun 27, 2025

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis
08:32

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Published on: February 14, 2022

7.7K

Light-induced Extracellular Vesicle Adsorption.

Colin L Hisey, Xilal Y Rima, Jacob Doon-Ralls

    Biorxiv : the Preprint Server for Biology
    |May 7, 2024
    PubMed
    Summary
    This summary is machine-generated.

    A new technique called Light-induced Extracellular Vesicle Adsorption (LEVA) enables label-free, high-resolution micropatterning of extracellular vesicles (EVs). This powerful tool advances the study of EVs and other particles for diverse applications.

    More Related Videos

    Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord
    09:01

    Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord

    Published on: May 23, 2021

    3.6K
    Imaging of Extracellular Vesicles by Atomic Force Microscopy
    10:11

    Imaging of Extracellular Vesicles by Atomic Force Microscopy

    Published on: September 11, 2019

    13.3K

    Related Experiment Videos

    Last Updated: Jun 27, 2025

    Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis
    08:32

    Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

    Published on: February 14, 2022

    7.7K
    Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord
    09:01

    Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord

    Published on: May 23, 2021

    3.6K
    Imaging of Extracellular Vesicles by Atomic Force Microscopy
    10:11

    Imaging of Extracellular Vesicles by Atomic Force Microscopy

    Published on: September 11, 2019

    13.3K

    Area of Science:

    • Biotechnology
    • Cell Biology
    • Materials Science

    Background:

    • Extracellular vesicles (EVs) play crucial roles in health and disease, interacting dynamically with the extracellular matrix.
    • Current methods lack label-free, high-resolution, and tunable platforms for creating high-fidelity EV micropatterns.
    • Studying matrix- and surface-bound EVs is essential for understanding their biological functions.

    Conclusions:

    • LEVA significantly advances the study of matrix- and surface-bound EVs and particles.
    • This platform is expected to spur innovation in diagnostics, biomimetics, immunoengineering, and therapeutic screening.
    • LEVA offers a versatile solution for researchers across multiple scientific disciplines.