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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K

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Updated: Jun 27, 2025

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Sample multiplexing for retinal single-cell RNA-sequencing.

Justin Ma, Ting Kuan Chu, Maria Polo Prieto

    Biorxiv : the Preprint Server for Biology
    |May 7, 2024
    PubMed
    Summary

    Sample multiplexing enhances single-cell RNA sequencing (scRNA-seq) precision for rare cell populations. This method tags samples with unique barcodes, enabling accurate analysis of cell types and variations across experiments.

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    Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
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    Area of Science:

    • Genomics
    • Molecular Biology
    • Neuroscience

    Background:

    • Characterizing rare cell populations with microfluidic single-cell RNA sequencing (scRNA-seq) is challenging.
    • Current enrichment and pooling methods obscure sample origin and phenotypic data, especially in high-variation experiments.

    Conclusions:

    • Sample multiplexing is a valuable method for increasing the precision of scRNA-seq analysis.
    • This technique is particularly useful for studies involving rare cell populations, disease models, and human samples.