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Hsa_circ_0051908 Promotes Hepatocellular Carcinoma Progression by Regulating the Epithelial-Mesenchymal Transition
Yinbing Wu1, Huafei Tang1, Shuzhong Cui1
1Department of Hepatobiliary Surgery, Affiliated Cancer Hospital and Institute of Guangzhou Medical University, Guangzhou, China.
Analytical Cellular Pathology (Amsterdam)
|May 8, 2024
Summary
Hsa_circ_0051908 promotes hepatocellular carcinoma (HCC) progression by enhancing cell proliferation and migration. Silencing this circular RNA inhibits tumor growth and may offer a new therapeutic strategy for HCC.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Hepatocellular carcinoma (HCC) is a prevalent malignancy with complex molecular underpinnings.
- Circular RNAs (circRNAs) are emerging as critical regulators in various cancers, including HCC.
Purpose of the Study:
- To investigate the role and mechanism of hsa_circ_0051908 in hepatocellular carcinoma (HCC) progression.
- To evaluate the potential of hsa_circ_0051908 as a therapeutic target for HCC.
Main Methods:
- Quantitative real-time PCR (RT-qPCR) for expression analysis.
- Cellular assays (CCK-8, EdU, TUNEL, flow cytometry, transwell) for proliferation, apoptosis, invasion, and migration.
- Western blotting for molecular mechanism investigation.
- In vivo tumor growth studies.
Main Results:
- Hsa_circ_0051908 expression was upregulated in HCC tissues and cell lines.
- Knockdown of hsa_circ_0051908 suppressed HCC cell proliferation, migration, and invasion while increasing apoptosis.
- Silencing hsa_circ_0051908 affected epithelial-mesenchymal transition (EMT) markers (vimentin, Snail, E-cadherin).
- In vivo, hsa_circ_0051908 inhibition significantly reduced tumor growth.
Conclusions:
- Hsa_circ_0051908 promotes HCC progression, partly via mediating EMT.
- Hsa_circ_0051908 represents a potential therapeutic target for hepatocellular carcinoma treatment.
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