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Related Experiment Videos

DR5-associated DC molecules carry three different allospecificities.

N Tanigaki, R Tosi, D Centis

    Human Immunology
    |January 1, 1985
    PubMed
    Summary

    A new antibody, 8w670, identifies a novel specificity called DC5. This specificity is found on DC molecules, distinct from previously known supertypic specificities, and is associated with DR5 and 8w13 positive cases.

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    Characterization of a new HLA-DRB1*01 allele (HLA-DRB1*010203) in a Caucasian Italian family by using sequence-based typing.

    Tissue antigens·2004

    Area of Science:

    • Immunology
    • Molecular Biology
    • Cell Surface Antigens

    Background:

    • Human leukocyte antigen (HLA) class II molecules play a crucial role in immune responses.
    • DR, BR, and DC molecules are subtypes of HLA class II antigens with distinct expression patterns.
    • Characterizing novel specificities on these molecules is essential for understanding immune function and disease association.

    Purpose of the Study:

    • To characterize the specificity of antiserum 8w670.
    • To determine the molecular expression of the 8w670-defined specificity on Ia molecules.
    • To investigate the relationship between the 8w670 specificity and known HLA class II supertypic specificities.

    Main Methods:

    • Direct binding assays using Ia preparations from LG38 cells.

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  • Binding inhibition assays to determine specificity.
  • Sequential binding analysis to identify co-expressed specificities.

    • Main Results:

    • Antiserum 8w670 reacted with approximately 40% of Ia molecules in a preparation enriched for DC molecules.
    • The 8w670 specificity was present in 100% of DR5 and 8w13 positive cases, and 23% of DR4 positive cases.
    • Sequential binding analysis confirmed the 8w670 specificity is on the same molecules carrying DC beta 4 and DC alpha 3 specificities.

    Conclusions:

    • Antiserum 8w670 defines a novel specificity, designated DC5.
    • DC5 is expressed on DC molecules and is associated with DR5 and 8w13.
    • The DC5 specificity is distinct from previously identified supertypic specificities but co-localizes with DC beta 4 and DC alpha 3.