Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

4.7K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.7K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.2K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Therapeutic TG2 inhibition reverses systemic multiomic dysregulation in celiac disease.

BMC medicine·2026
Same author

A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells.

Angewandte Chemie (International ed. in English)·2025
Same author

RNA-binding proteins mediate the maturation of chromatin topology during differentiation.

Nature cell biology·2025
Same author

MINFLUX: μs and nm precision 3D tracking of dynamic lipid mobility on nanoparticles.

Nanoscale·2025
Same author

Transcriptomic analysis of intestine following administration of a transglutaminase 2 inhibitor to prevent gluten-induced intestinal damage in celiac disease.

Nature immunology·2024
Same author

Build and operation of a custom 3D, multicolor, single-molecule localization microscope.

Nature protocols·2024

Related Experiment Video

Updated: Jun 26, 2025

Major Components of the Light Microscope
08:08

Major Components of the Light Microscope

Published on: July 30, 2008

22.8K

Setting up a light microscopy core facility: Facility design.

Timo Zimmermann1

  • 1EMBL Imaging Centre, European Molecular Biology Laboratory, Heidelberg, Germany.

Journal of Microscopy
|May 8, 2024
PubMed
Summary

Proper infrastructure setup is crucial for light microscopy core facilities. Key considerations include location, room design, and environmental factors like light, temperature, and vibrations for optimal microscope performance.

Keywords:
microscope room planningtemperaturevibrations

More Related Videos

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

2.0K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.9K

Related Experiment Videos

Last Updated: Jun 26, 2025

Major Components of the Light Microscope
08:08

Major Components of the Light Microscope

Published on: July 30, 2008

22.8K
Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

2.0K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.9K

Area of Science:

  • Microscopy
  • Core Facility Management
  • Laboratory Infrastructure

Background:

  • Successful operation of light microscopy core facilities relies on robust infrastructure.
  • Initial setup, including location and environmental controls, is critical for performance.

Purpose of the Study:

  • To outline essential infrastructure considerations for establishing a light microscopy core facility.
  • To detail environmental factors impacting microscope performance and longevity.

Main Methods:

  • Review of best practices in laboratory design and infrastructure planning.
  • Identification of critical environmental parameters for light microscopy.

Main Results:

  • Location selection and room planning are foundational.
  • Environmental factors such as ambient light, temperature stability, and vibration control are paramount.
  • Adequate basic installations are necessary for reliable microscope operation.

Conclusions:

  • Strategic infrastructure planning is essential for the successful establishment and operation of light microscopy core facilities.
  • Controlling environmental factors ensures optimal performance and extends the lifespan of sensitive microscopy equipment.