Electrochemical Immunoassay Platform for Human Monkeypox Virus Detection

Area of Science:

• Biomedical Engineering
• Biosensor Technology
• Virology

Background:

• Monkeypox virus (MPXV) poses a significant public health threat.
• Early and accurate detection of MPXV is crucial for disease control.
• MPXV internal membrane protein A29 is a potential diagnostic target.

Purpose of the Study:

• To develop a selective impedimetric biosensor for MPXV A29 protein detection.
• To investigate the interaction between heparan sulfate and MPXV A29 protein for biosensing.
• To evaluate the analytical performance and applicability of the developed biosensor.

Main Methods:

• Immobilization of heparan sulfate onto a gold screen-printed electrode.
• Monitoring the interaction with MPXV A29 protein using electrochemical impedance spectroscopy.
• Optimization of experimental parameters and characterization of the immunosensor.

Main Results:

• The developed MPXV immunosensor demonstrated a linear detection range from 2.0 to 50 ng mL⁻¹.
• A low detection limit of 2.08 ng mL⁻¹ and quantification limit of 6.28 ng mL⁻¹ were achieved.
• High selectivity and good recovery rates were observed in real serum samples.

Conclusions:

• The developed impedimetric biosensor offers a sensitive and selective method for MPXV A29 detection.
• This biosensor shows potential for rapid and reliable diagnosis of monkeypox infections.
• The findings contribute to the development of advanced diagnostic tools for viral diseases.