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Updated: Jun 25, 2025

A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line

A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line

Published on: December 30, 2012

Rapid and practical colorimetric biosensor for leishmaniasis diseases.

Göksu Can¹, Amira Chouihi², Mohamed Fethi Diouani²

¹Mugla Sitki Kocman University, Faculty of Science, Chemistry Department, Kotekli-Mugla, Turkey.

Diagnostic Microbiology and Infectious Disease

|May 20, 2024

Summary

A new colorimetric biosensor detects Leishmania major surface protease (Gp63) antibodies using gold nanoparticles. This sensitive assay offers rapid detection of anti-Gp63, crucial for diagnosing leishmaniasis.

Keywords:

Colorimetric biosensor Gold nanoparticles Kinetoplastid membrane protein-11 (KMP-11)Leishmania glycoprotein 63 (Gp63)Leishmania parasite Leishmaniasis disease

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Published on: March 16, 2018

Area of Science:

Biomedical Engineering
Nanotechnology
Parasitology

Background:

Leishmaniasis diagnosis requires sensitive and specific methods.
The surface protease (Gp63) of Leishmania major is a key diagnostic target.
Gold nanoparticles (AuNPs) offer unique optical properties for biosensing.

Purpose of the Study:

To develop a novel colorimetric biosensor for detecting antibodies against Leishmania major surface protease (Gp63).
To utilize gold nanoparticles (AuNPs) as a colorimetric indicator for antibody detection.
To establish the sensitivity and reliability of the developed biosensor for leishmaniasis diagnosis.

Main Methods:

Conjugation of kinetoplastid membrane protein-11 (KMP-11) to gold nanoparticles (AuNPs).
Colorimetric detection of anti-Gp63 antibodies via AuNP aggregation/dispersion.
UV-vis spectroscopy and visual color change analysis for detection.

Main Results:

The biosensor detected anti-Gp63 antibodies at dilutions up to 1:640.
A distinct color change from red/pink to purple/violet indicated positive results.
High recovery rates (95.3%–103.1%) and low relative standard deviation (1.29%) were achieved.

Conclusions:

The developed gold nanoparticle-based colorimetric biosensor is a sensitive and reliable tool for detecting anti-Gp63 antibodies.
This assay shows potential for rapid and accessible diagnosis of leishmaniasis.
The biosensor demonstrated effective detection in crude antigen and serum samples.