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Enzyme-Linked Immunosorbent Assay01:33

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Electrochemical Immunoassay Platform for Human Monkeypox Virus Detection.

Göksu Can1, Benay Perk1, Burak Ekrem Çitil2

  • 1Faculty of Science, Chemistry Department, Mugla Sitki Kocman University, Kotekli, Mugla 48000, Turkey.

Analytical Chemistry
|May 10, 2024
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Summary

A novel impedimetric biosensor was developed for detecting the monkeypox virus (MPXV) A29 protein. This biosensor utilizes heparan sulfate interaction for selective MPXV detection in serum samples.

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Area of Science:

  • Biomedical Engineering
  • Biosensor Technology
  • Virology

Background:

  • Monkeypox virus (MPXV) poses a significant public health threat.
  • Early and accurate detection of MPXV is crucial for disease control.
  • MPXV internal membrane protein A29 is a potential diagnostic target.

Purpose of the Study:

  • To develop a selective impedimetric biosensor for MPXV A29 protein detection.
  • To investigate the interaction between heparan sulfate and MPXV A29 protein for biosensing.
  • To evaluate the analytical performance and applicability of the developed biosensor.

Main Methods:

  • Immobilization of heparan sulfate onto a gold screen-printed electrode.
  • Monitoring the interaction with MPXV A29 protein using electrochemical impedance spectroscopy.
  • Optimization of experimental parameters and characterization of the immunosensor.

Main Results:

  • The developed MPXV immunosensor demonstrated a linear detection range from 2.0 to 50 ng mL⁻¹.
  • A low detection limit of 2.08 ng mL⁻¹ and quantification limit of 6.28 ng mL⁻¹ were achieved.
  • High selectivity and good recovery rates were observed in real serum samples.

Conclusions:

  • The developed impedimetric biosensor offers a sensitive and selective method for MPXV A29 detection.
  • This biosensor shows potential for rapid and reliable diagnosis of monkeypox infections.
  • The findings contribute to the development of advanced diagnostic tools for viral diseases.