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Viral Nanoparticles for In vivo Tumor Imaging
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Receptor Targeting Using Copolymer-Modified Gold Nanoparticles for pCMV-Luc Gene Delivery to Liver Cancer Cells In

Mkhuseli Zenze1, Moganavelli Singh1

  • 1Nano-Gene and Drug Delivery Laboratory, Discipline of Biochemistry, University of KwaZulu-Natal, Private Bag X54001, Durban 4000, South Africa.

International Journal of Molecular Sciences
|May 11, 2024
PubMed
Summary
This summary is machine-generated.

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Researchers developed targeted gold nanoparticles (AuNPs) for liver gene therapy. These safe, biocompatible AuNPs effectively delivered genes to liver cells, showing potential for treating liver disorders.

Area of Science:

  • Nanomedicine
  • Gene Therapy
  • Hepatocyte Targeting

Background:

  • Targeted gene delivery to hepatocytes is crucial for treating liver diseases.
  • Non-viral methods, particularly using nanoparticles, are explored for gene therapy.
  • Gold nanoparticles (AuNPs) offer biocompatibility for nanomedical applications.

Purpose of the Study:

  • To formulate and characterize novel lactobionic acid-targeted gold nanoparticles (AuNPs) for hepatocyte-specific gene delivery.
  • To evaluate the physicochemical properties, DNA complexation, and cytotoxicity of the AuNPs.
  • To assess the efficacy of targeted AuNPs in enhancing gene expression in liver cells.

Main Methods:

  • Synthesis and polymer coating (chitosan, PEG) of AuNPs.
  • Functionalization with lactobionic acid (LA) for targeting.
Keywords:
chitosangene expressiongold nanoparticleslactobionic acidliver targetingpolyethylene glycol

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  • Physicochemical characterization (size, morphology).
  • Complexation and protection of plasmid DNA (pCMV-Luc).
  • In vitro cytotoxicity and transgene expression assays in HepG2, HEK293, and Caco-2 cells.

    • Main Results:

    • Spherical, monodispersed AuNPs (70-250 nm) were successfully synthesized and coated.
    • AuNPs effectively bound, compacted, and protected plasmid DNA.
    • AuNPs exhibited good biocompatibility (cell viability >70%) across tested cell lines.
    • LA-targeted AuNPs significantly enhanced luciferase gene expression (five-fold increase) in HepG2 cells compared to non-targeted AuNPs.

    Conclusions:

    • The developed LA-targeted AuNPs are safe and effective for delivering genes to hepatocytes.
    • These nanoparticles show significant potential as delivery vehicles for liver-directed gene therapy.
    • The receptor-mediated targeting strategy enhances therapeutic efficacy for liver-specific disorders.