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Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
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Faecal Volatile Organic Compound Analysis in De Novo Paediatric Inflammatory Bowel Disease by Gas Chromatography-Ion

Eva Vermeer1,2,3, Jasmijn Z Jagt1,2,3, Trenton K Stewart4

  • 1Department of Paediatric Gastroenterology, Emma Children's Hospital, Amsterdam University Medical Centre, 1105 AZ Amsterdam, The Netherlands.

Sensors (Basel, Switzerland)
|May 11, 2024
PubMed

Insights

Faecal volatile organic compound (VOC) profiles distinguish paediatric inflammatory bowel disease (IBD) patients from controls. However, baseline VOC patterns did not predict treatment response in these young IBD patients.

Area of Science:

  • Microbiology
  • Gastroenterology
  • Metabolomics

Background:

  • Gut microbiota and metabolites differ in inflammatory bowel disease (IBD).
  • Volatile organic compounds (VOCs) are microbial metabolites with diagnostic potential.
  • Paediatric IBD patient data on VOC profiles and treatment response is limited.

Purpose of the Study:

  • To compare faecal VOC profiles between paediatric IBD patients and controls with gastrointestinal symptoms (CGIs).
  • To determine if baseline VOC profiles can predict treatment response in paediatric IBD patients.

Main Methods:

  • Faecal samples were collected from de novo, therapy-naïve paediatric IBD patients and CGIs (aged 4-17).
  • Gas chromatography-ion mobility spectrometry (GC-IMS) was used for VOC analysis.
  • Treatment response was defined by clinical scores without treatment escalation.

Main Results:

  • Paediatric IBD patients showed distinct faecal VOC profiles compared to CGIs (AUC 0.71, p<0.001).
  • Distinct VOC profiles were observed in both Crohn's disease (AUC 0.75) and ulcerative colitis (AUC 0.67) patients.
  • No significant difference in baseline VOC profiles was found between responders and non-responders (AUC 0.70, p=0.1).

Conclusions:

  • Faecal VOC profiles significantly differentiate paediatric IBD patients from CGIs.
  • Current VOC analysis does not predict treatment response in paediatric IBD patients.

Keywords:
faecal volatile organic compoundsgas chromatography–ion mobility spectrometrypaediatric inflammatory bowel disease

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