Dual stimuli-responsive upconversion nanoparticle-poly-N-isopropylacrylamide/DNA core-shell microgels

Area of Science:

• Materials Science
• Nanotechnology
• Biotechnology

Background:

• Upconversion nanoparticles (UCNPs) offer unique near-infrared (NIR) responsive properties.
• Poly-N-isopropylacrylamide (pNIPAM) is a well-known thermosensitive polymer.
• DNA nanotechnology enables precise control over molecular functions.

Purpose of the Study:

• To prepare stimuli-responsive core-shell microgels integrating UCNPs, pNIPAM, and DNA.
• To achieve tunable microgel sizes and programmable functionalities.
• To enable smart regulation of DNA activity using external stimuli.

Main Methods:

• Synthesis of UCNP-pNIPAM/DNA core-shell microgels.
• Characterization of microgel size and morphology.
• Assessment of DNA loading efficiency and activity.
• Evaluation of NIR and temperature-triggered DNA regulation.

Main Results:

• Successfully prepared UCNP-pNIPAM/DNA core-shell microgels with tunable sizes.
• Achieved high DNA activity and loading efficiency within the microgels.
• Demonstrated simultaneous regulation of DNA activity by NIR illumination and temperature.
• UCNP cores provide NIR responsiveness, while pNIPAM shells offer thermosensitivity.

Conclusions:

• The developed microgels exhibit excellent stimuli-responsive behavior for controlled DNA release and activity.
• These smart microgels hold promise for advanced applications in drug delivery and diagnostics.
• The combination of UCNPs, pNIPAM, and DNA offers a versatile platform for functional nanomaterials.