CD22 blockade aggravates EAE and its role in microglia polarization
Weiwei Xiang1, Kan Wang1, Lu Han1
1Department of Neurology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
CNS Neuroscience & Therapeutics
|May 13, 2024
Summary
CD22 blockade worsens experimental autoimmune encephalomyelitis (EAE) in mice by promoting M1 microglial polarization, suggesting CD22 is protective against this neuroinflammatory disease.
Area of Science:
- Neuroimmunology
- Microglial biology
Background:
- Multiple sclerosis (MS) is a neuroinflammatory demyelinating disease.
- Microglia play a role in MS pathogenesis, but key regulatory molecules remain unknown.
Purpose of the Study:
- To investigate the role of CD22 in microglial activation and experimental autoimmune encephalomyelitis (EAE).
Main Methods:
- CD22 expression in microglia was analyzed in EAE mice.
- In vitro studies examined lipopolysaccharide-induced CD22 upregulation and CD22 blockade effects on microglial polarization.
- In vivo studies assessed the impact of CD22 blockade on EAE severity.
Main Results:
- Microglial CD22 expression increased in EAE mice.
- CD22 blockade modulated microglial polarization towards the M1 phenotype.
- CD22 blockade aggravated EAE and promoted M1 microglial polarization in vivo.
Conclusions:
- CD22 appears to be protective against EAE.
- CD22 plays a critical role in maintaining immune homeostasis in EAE models.
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