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Miniaturized matrix solid-phase dispersion assisted by deep eutectic solvent for acrylamide determination in bread

Susanna Della Posta1, Anna Maria Ascrizzi1, Giorgia Pietrangeli1

  • 1Department of Science and Technology for Sustainable Development and One Health, Campus Bio-Medico University of Rome, Via Alvaro del Portillo 21, 00128, Rome, Italy.

Analytical and Bioanalytical Chemistry
|May 13, 2024
PubMed
Summary
This summary is machine-generated.

A new method uses green deep eutectic solvents (DES) with matrix solid-phase dispersion (MSPD) to efficiently extract acrylamide from baked goods. This approach reduces organic solvent use while ensuring accurate acrylamide determination for food safety.

Keywords:
AcrylamideDeep eutectic solventGreen extractionHPLC–MSMatrix solid-phase dispersion

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Area of Science:

  • Food Chemistry
  • Analytical Chemistry
  • Green Chemistry

Background:

  • Acrylamide determination in baked foods is crucial for mitigating potential carcinogenic risks.
  • Matrix solid-phase dispersion (MSPD) is a common technique for acrylamide extraction, enhancing analyte yield.
  • Deep eutectic solvents (DES) offer a greener alternative, improving extraction efficiency and reducing organic solvent consumption.

Purpose of the Study:

  • To develop a miniaturized MSPD extraction method for acrylamide determination in bread.
  • To incorporate a green DES into the MSPD process to enhance acrylamide extraction.
  • To validate the developed method for accuracy, precision, and sensitivity.

Main Methods:

  • A miniaturized matrix solid-phase dispersion (MSPD) extraction was optimized for bread samples.
  • Choline chloride-glycerol DES was added during the homogenization phase of MSPD.
  • Acrylamide was quantified using high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS).

Main Results:

  • The optimized procedure used a 1:1 (w/w) matrix-to-dispersing material ratio and 2 mL of methanol.
  • The addition of 50 μL of choline chloride-glycerol DES significantly improved extraction.
  • Method validation demonstrated high recoveries (≥90%), excellent precision (intra-day error ≤4%, inter-day error ≤6%), and low detection limits (LOD: 16 μg/kg, LOQ: 35 μg/kg).

Conclusions:

  • The developed miniaturized MSPD extraction assisted by DES is an effective and green method for acrylamide determination in bread.
  • This approach offers a viable alternative to existing methods, providing high performance and adhering to green chemistry principles.
  • The method ensures reliable acrylamide quantification, contributing to food safety and risk assessment.