Related Experiment Video
Updated: Apr 30, 2026
09:42
High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue
Published on: April 30, 2019
10.9K
Histopathological Analysis of Brains from Dogs Infected with Canine Distemper Virus
José Manuel Verdes1, Camila Larrañaga2, Belén Varela2
1Department of Pathobiology, Universidad de la República, Montevideo, Uruguay. jmverdes@fvet.edu.uy.
Methods in Molecular Biology (Clifton, N.J.)
|May 14, 2024
Summary
Histopathology and immunohistochemistry reveal typical brain lesions in canine distemper virus (CDV)-infected dogs. This study highlights canine distemper as a valuable natural model for studying morbillivirus infections.
Area of Science:
- Veterinary Pathology
- Neuroscience
- Virology
Background:
- Canine distemper virus (CDV) causes significant neurological disease in domestic dogs.
- Understanding the neuropathogenesis of CDV is crucial for developing effective treatments and preventative measures.
Purpose of the Study:
- To utilize conventional histology and immunohistochemistry to characterize brain lesions in CDV-infected dogs.
- To evaluate the utility of canine distemper as a natural model for studying morbillivirus infections.
Main Methods:
- Conventional Hematoxylin and Eosin (H&E) staining for general histopathology.
- Luxol Fast Blue (LFB) staining to assess demyelination.
- Immunohistochemistry (IHC) targeting CDV to detect viral particles in affected brain tissues.
Main Results:
- Histopathological analysis confirmed characteristic brain lesions associated with CDV infection.
- Demyelination was evident using LFB staining.
- Immunohistochemistry successfully localized CDV antigens within the affected neural tissues.
Conclusions:
- Histopathological examination of canine brains is a robust method for evaluating CDV-induced lesions.
- Canine distemper serves as a relevant natural model for investigating the pathogenesis of CDV and other morbilliviruses, such as measles virus.
Related Concept Videos
01:26Encephalitis ll: Pathophysiology
22
Encephalitis is inflammation of the brain parenchyma caused by direct viral invasion or immune-mediated mechanisms triggered by infections or tumors. Both processes lead to neuronal injury, disrupted neurotransmission, and diverse neurological symptoms, often with overlapping clinical and pathological features.Autoimmune EncephalitisIn autoimmune encephalitis, antibodies target neuronal antigens on cell surfaces, synapses, or within neurons. A key example is anti-NMDAR encephalitis, which can...
22
01:26Brain Abscess l: Introduction
21
A brain abscess is a focal, intracerebral infection characterized by a localized collection of pus within the brain parenchyma, resulting from microbial invasion and the body’s inflammatory response. It progresses through stages: early and late cerebritis, followed by early and late capsule formation, reflecting tissue destruction, immune response, and eventual encapsulation.Etiology and PathogenesisCausative organisms vary with source and host factors, often involving polymicrobial...
21