Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enzymatic Deacetylation as a Selective Strategy for <i>O</i>-GlcNAc Identification.

Analytical chemistry·2026
Same author

Malaria Public Health Status: Global Context and Update on Gulf Cooperation Council Countries.

Advances in experimental medicine and biology·2026
Same author

Author Correction: High-throughput discovery of fluoroprobes that recognize amyloid fibril polymorphs.

Nature chemistry·2026
Same author

Intracellular potassium levels orchestrate circadian rhythmicity and cell division.

Nature communications·2026
Same author

Emergence of specific binding and catalysis from a designed generalist binding protein.

Nature chemistry·2026
Same author

Solvatochromic BODIPYs with Polarity-Independent Absorption for Functional Super-resolution Imaging of Live Cell Membranes.

Chemical & biomedical imaging·2026

Related Experiment Video

Updated: Jun 26, 2025

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
13:26

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

Published on: September 13, 2014

61.7K

Protein-adaptive differential scanning fluorimetry using conformationally responsive dyes.

Taiasean Wu1,2, Joshua C Yu1, Arundhati Suresh1

  • 1Department of Pharmaceutical Chemistry, University of California, San Francisco, CA, USA.

Nature Biotechnology
|May 14, 2024
PubMed
Summary
This summary is machine-generated.

A new protein-adaptive differential scanning fluorimetry (paDSF) platform expands protein stability analysis. This method significantly increases compatibility with diverse proteins, enabling new biological insights.

More Related Videos

Author Spotlight: Streamlining PCR Methods for Enhanced Accessibility and Efficiency
05:30

Author Spotlight: Streamlining PCR Methods for Enhanced Accessibility and Efficiency

Published on: March 1, 2024

478
Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery
06:26

Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery

Published on: May 16, 2021

4.8K

Related Experiment Videos

Last Updated: Jun 26, 2025

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
13:26

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

Published on: September 13, 2014

61.7K
Author Spotlight: Streamlining PCR Methods for Enhanced Accessibility and Efficiency
05:30

Author Spotlight: Streamlining PCR Methods for Enhanced Accessibility and Efficiency

Published on: March 1, 2024

478
Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery
06:26

Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery

Published on: May 16, 2021

4.8K

Area of Science:

  • Biochemistry
  • Biophysics
  • Molecular Biology

Background:

  • Differential scanning fluorimetry (DSF) measures protein thermal stability using fluorescent dyes.
  • Current DSF methods face limitations due to protein-dye incompatibilities, restricting their application.
  • There is a need for broader compatibility in DSF assays for diverse protein studies.

Purpose of the Study:

  • To develop a novel protein-adaptive differential scanning fluorimetry (paDSF) platform.
  • To overcome existing limitations in protein-dye compatibility for DSF.
  • To enable robust protein stability and dynamics analysis across a wider range of proteins.

Main Methods:

  • Development of a protein-adaptive DSF (paDSF) platform incorporating the 'Aurora' dye library.
  • Implementation of a streamlined procedure for identifying optimal protein-dye pairs.
  • Application of paDSF to a diverse set of 70 proteins, including viral proteins.

Main Results:

  • paDSF demonstrated high compatibility, successfully assaying 94% (66 of 70) of tested proteins.
  • The platform enabled the development of new DSF assays for 66 functionally diverse proteins.
  • paDSF revealed previously inaccessible biological processes, such as O-GlcNAc transferase interdomain allostery.

Conclusions:

  • The paDSF platform significantly expands the utility of DSF for protein stability and dynamics studies.
  • The 'Aurora' dye library and paDSF procedure offer a generalizable tool for protein interrogation.
  • This approach facilitates the study of protein behavior in various biological contexts, including infectious diseases.