Dialysis-induced transformation of mouse submandibular androgen-receptor

Dialysis induced transformation of cytosol androgen receptor from mouse submandibular gland. On DEAE-Sephacel chromatography, this dialyzed [3H]methyltrienolone receptor complex was eluted at 0.10 M KCl which was lower than 0.25 M KCl required to elute the nontransformed androgen receptor complex, but higher than 0.05 M KCl required to elute the heat transformed receptor. On glycerol density gradient centrifugation, the dialysis transformed receptor complex shifted its sedimentation coefficient to 6 S from 8 S of the nontransformed condition, whereas the heat transformed receptor was sedimented at 4 S. Molybdate inhibited the dialysis-induced transformation on DEAE-Sephacel chromatography. The charge and the molecular size of dialysis-induced transformed receptor complex were different from those of heat-induced transformed receptor complex.