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High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells

High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells

Published on: January 7, 2019

Reconstituting membrane fission using a high content and throughput assay.

Uma Swaminathan¹, Thomas J Pucadyil¹

¹Indian Institute of Science Education and Research, Pune, India.

Biochemical Society Transactions

|May 15, 2024

Summary

Supported membrane template (SMrT) assays offer high-throughput analysis of protein-mediated membrane fission. These assays reveal fission intermediates and facilitate protein function annotation, advancing cell biology research.

Keywords:

biochemical assays dynamin superfamily fluorescence microscopy membrane tubes reconstitution

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Last Updated: Jun 26, 2025

High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells

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Published on: January 7, 2019

Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.

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A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics

A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics

Published on: September 2, 2020

Area of Science:

Cell Biology
Biophysics
Biochemistry

Background:

Protein-mediated membrane fission is crucial for cellular processes.
Traditional in vitro tether assays offer low throughput.
A need exists for high-content, high-throughput membrane fission analysis.

Purpose of the Study:

To review the utility of supported membrane template (SMrT) assays for studying membrane fission.
To highlight SMrTs' ability to provide insights into fission pathways.
To discuss SMrTs' application in annotating protein functions related to membrane fission.

Main Methods:

Review of existing literature on membrane fission assays.
Focus on the supported membrane template (SMrT) assay system.
Analysis of SMrT's advantages in throughput and content.

Main Results:

SMrTs provide high-throughput monitoring of numerous fission events.
SMrTs yield valuable information on intermediates in the membrane fission pathway.
SMrTs are adaptable for annotating protein functions in membrane fission.

Conclusions:

SMrTs represent a significant advancement in studying membrane fission.
The SMrT assay system enhances understanding of fission mechanisms.
SMrTs facilitate functional characterization of proteins involved in membrane dynamics.