Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

974
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
974
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

760
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
760
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

655
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
655
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

1.3K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.3K
Mass Spectrometers01:16

Mass Spectrometers

5.4K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An Ultra-High Throughput Hydrogen-Deuterium Exchange Workflow Using Acoustic Ejection Mass Spectrometry for Studying Peptide Solution Structural Conformation.

Analytical chemistry·2026
Same author

Evaluating the Utility and Constraints of the Competitive Counter Flow (CCF) Assay in OATP1B1 Substrate Profiling.

The AAPS journal·2026
Same author

Comparative study of absorption prediction using human intestinal organoid-derived cells in static and flow conditions.

Drug metabolism and disposition: the biological fate of chemicals·2026
Same author

Molnupiravir is not a selective CES2 probe substrate: in vitro evidence for CES1 involvement.

Drug metabolism and disposition: the biological fate of chemicals·2026
Same author

Accelerating the discovery of MET inhibitors powered by high-throughput hit identification.

Bioanalysis·2025
Same author

Rapid Screening and Prioritization of Culture Conditions for Natural Product Discovery using the Liquid Microjunction Surface Sampling Probe.

Journal of the American Society for Mass Spectrometry·2025
Same journal

Machine Learning-Assisted Label-Free SERS Decoding of Mitochondrial Molecular Dynamics in Ovarian Granulosa Cells during Aging.

Analytical chemistry·2026
Same journal

Revealing the Regulatory Interplay of NHE1 mRNA and Na<sup>+</sup> in Cancer Cells Using a DNA Nanosensor.

Analytical chemistry·2026
Same journal

Towards Cellular Resolution of Tryptic Peptides in Tissue Sections by MALDI MS Imaging: A Focus on Enzyme Application and Reproducibility.

Analytical chemistry·2026
Same journal

Bioinspired Bilayer Hydrogel Colorimetric Sensor Array for Low-Temperature Food Freshness Analysis.

Analytical chemistry·2026
Same journal

Quartz Crystal Microbalance-Based Point-of-Care Testing Systems: Principles, Device Design, and Applications.

Analytical chemistry·2026
Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 26, 2025

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
09:24

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: December 18, 2020

5.6K

High-Throughput Compound Quality Assessment with High-Mass-Resolution Acoustic Ejection Mass Spectrometry: An

Alandra Quinn1, Gordana Ivosev2, Jefferson Chin1,3

  • 1Pfizer Global Research and Development, Groton, Connecticut 06340, United States.

Analytical Chemistry
|May 16, 2024
PubMed
Summary
This summary is machine-generated.

High-throughput compound quality control (QC) is vital for pharmacological screening. Acoustic ejection mass spectrometry (AEMS) coupled with a data processing toolkit enables rapid and reliable QC for large compound libraries.

More Related Videos

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water
09:43

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water

Published on: February 20, 2013

12.0K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.0K

Related Experiment Videos

Last Updated: Jun 26, 2025

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
09:24

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: December 18, 2020

5.6K
Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water
09:43

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water

Published on: February 20, 2013

12.0K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.0K

Area of Science:

  • Analytical Chemistry
  • Pharmacology
  • Biotechnology

Background:

  • Reliable compound libraries are crucial for accurate pharmacological screening results.
  • Current quality control (QC) methods using liquid chromatography (LC) lack the throughput for large-scale libraries.
  • Acoustic ejection mass spectrometry (AEMS) offers a high-throughput analytical solution for diverse chemical structures.

Purpose of the Study:

  • To present the application of a high-resolution AEMS system for high-throughput compound QC.
  • To introduce an automated data processing toolkit for efficient analysis of large compound sets.
  • To demonstrate the combined system's capability for rapid and reliable quality assessment of compound libraries.

Main Methods:

  • Utilized a high-resolution acoustic ejection mass spectrometry (AEMS) system for compound analysis.
  • Developed an automated data processing toolkit for quantitative and qualitative assessment.
  • Implemented spectral similarity comparison against a reference library within the toolkit.

Main Results:

  • The AEMS system effectively processed large sample sets at high throughput.
  • The automated toolkit enabled efficient purity calculations and qualitative assessments.
  • The integrated system successfully addressed the analytical bottleneck in compound QC.

Conclusions:

  • High-resolution AEMS combined with an automated data processing toolkit provides a rapid and reliable solution for large-scale compound library QC.
  • This integrated approach significantly enhances the efficiency of pharmacological screening by ensuring library quality.
  • The developed toolkit facilitates comprehensive analysis, including purity and spectral similarity, crucial for compound validation.