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Creating 3D constructs with cranial neural crest-derived cell lines using a bio-3D printer.

Masahide Taguchi1, Shohei Yoshimoto2, Kanako Suyama1

  • 1Section of Pediatric Dentistry, Department of Oral Growth and Development, Fukuoka Dental College, Fukuoka, Japan.

Journal of Oral Biosciences
|May 16, 2024
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Summary

Researchers developed a novel 3D construct using bio-3D printing for regenerative medicine. This model mimics the dentin-pulp complex, showing polarized expression of key extracellular matrix proteins essential for studying tooth development (odontogenesis).

Keywords:
3D constructsBio-3D printerSpheroidTenascin C

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Area of Science:

  • Regenerative Medicine
  • Bioprinting
  • Developmental Biology

Background:

  • Bio-three-dimensional (bio-3D) printing advances regenerative medicine.
  • Three-dimensional (3D) constructs, like spheroids, utilize extracellular matrix proteins for physiologically relevant cell culturing.
  • A 3D construct model of the dentin-pulp complex is needed.

Purpose of the Study:

  • To create a functional 3D construct for modeling the dentin-pulp complex.
  • To evaluate the expression patterns of extracellular matrix proteins and cell proliferation in the 3D construct.

Main Methods:

  • Utilized O9-1 cells derived from mouse cranial neural crest cells.
  • Fabricated 3D constructs by adhering spheroid cultures onto a needle array using a bio-3D printer.
  • Examined expression of tenascin C and DMP1, and cell proliferation areas.

Main Results:

  • Tenascin C and DMP1 expression were significantly enhanced in spheroids versus 2D cultures.
  • Cell proliferation and tenascin C expression were localized to the outer layer in embryonic stem cell medium.
  • DMP1 expression was promoted in calcification-induction medium, with DMP1-positive cells in the outer layer, distinct from tenascin C.

Conclusions:

  • Extracellular matrix proteins tenascin C and DMP1 exhibited polarized expression in 3D constructs, mirroring dental papilla characteristics.
  • These 3D constructs demonstrate potential as artificial models for investigating odontogenesis.