Phytohabitans maris sp. nov., isolated from marine sediment

A novel actinobacterium, strain ZYX-F-186^T, was isolated from marine sediment sampled on Yongxing Island, Hainan Province, PR China. Based on the results of 16S rRNA gene sequence analysis, strain ZYX-F-186^T belongs to the genus Phytohabitans, with high similarity to Phytohabitans kaempferiae KK1-3^T (98.3 %), Phytohabitans rumicis K11-0047^T (98.1 %), Phytohabitans flavus K09-0627^T (98.1 %), Phytohabitans houttuyneae K11-0057^T (97.9 %), Phytohabitans suffuscus K07-0523^T (97.7 %), and Phytohabitans aurantiacus RD004123^T (97.7 %). Phylogenetic analysis of 16S rRNA gene sequences showed that the strain formed a single subclade in the genus Phytohabitans. The novel isolate contained meso-diaminopimelic acid, d-glutamic acid, glycine, d-alanine, and l-lysine in the cell wall. The whole-cell sugars were xylose, arabinose, ribose, and rhamnose. The predominant menaquinones were MK-9(H₈), MK-9(H₆), and MK-9(H₄). The characteristic phospholipids were phosphatidylethanolamine, phosphatidylinositol, phosphatidylmethylethanolamine, phosphatidylglycerol, and an unknown phospholipid. The major fatty acids (>5 %) were iso-C_16 : 0, anteiso-C_17 : 0, and iso-C_18 : 0. Genome sequencing showed a DNA G+C content of 71.9 mol%. Low average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity values demonstrated that strain ZYX-F-186^T could be readily distinguished from its closely related species. Based on its phylogenetic, chemotaxonomic, and physiological characteristics, strain ZYX-F-186^T represents a novel species of the genus Phytohabitans, for which the name Phytohabitans maris sp. nov. is proposed. The type strain is ZYX-F-186^T (=CGMCC 4.8025^T=CCTCC AA 2023025^T=JCM 36507^T).