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[Extracellular thermostable alpha-amylase from Bacillus licheniformis B7]
Zhonghua Minguo Wei Sheng Wu Ji Mian Yi Xue Za Zhi = Chinese Journal of Microbiology and Immunology
|February 1, 1985
Summary
Bacillus licheniformis B7 produces a thermostable alpha-amylase. Optimal conditions for enzyme production include 50°C, 7 days incubation, specific media supplements, and starch as a carbon source.
Area of Science:
- Microbiology
- Enzymology
- Biotechnology
Background:
- Thermostable alpha-amylases are crucial industrial enzymes.
- Bacillus licheniformis is a known source of valuable enzymes.
- Optimizing enzyme production is key for industrial applications.
Purpose of the Study:
- To isolate and identify a bacterium producing thermostable alpha-amylase.
- To determine optimal cultural conditions for alpha-amylase production by Bacillus licheniformis B7.
- To characterize the properties of the produced alpha-amylase.
Main Methods:
- Bacterial isolation from soil and identification.
- Optimization of cultural parameters (temperature, incubation time, carbon source, media supplements).
- Enzyme activity assays at varying temperatures and pH.
Main Results:
- Bacillus licheniformis B7 was identified as the enzyme producer.
- Optimal production occurred at 50°C with 2.5% starch, 0.6% yeast extract, 0.35% peptone, 0.2% Na-citrate, 0.008% CaCl2, and 0.05% FeCl3 over 7 days.
- The enzyme exhibited maximum activity at 90°C and pH 9.0.
Conclusions:
- Bacillus licheniformis B7 is a promising source of thermostable alpha-amylase.
- Specific cultural conditions significantly enhance enzyme yield.
- The characterized enzyme possesses properties suitable for high-temperature industrial processes.