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Updated: Jun 26, 2025

Shifting Zebrafish Lethal Skeletal Mutant Penetrance by Progeny Testing

Shifting Zebrafish Lethal Skeletal Mutant Penetrance by Progeny Testing

Published on: September 1, 2017

Deletion of Asb15b gene can lead to a significant decrease in zebrafish intermuscular bone.

Minghui Niu¹, Huamin Whang¹, Zhichao Wu¹

¹College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Fishery Germplasm Resources Exploration and Utilization, Ministry of Education, Shanghai 201306, China.

|May 16, 2024

Summary

The Asb15b gene is crucial for reducing intermuscular bones in fish. Gene editing in zebrafish significantly decreased bone formation, offering potential for aquaculture improvements.

Keywords:

Asb15b gene CRISPR/Cas9 Intermuscular bone Zebrafish

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Published on: December 13, 2013

Area of Science:

* Genetics and Developmental Biology
* Aquaculture and Fish Biology

Background:

* Intermuscular bones negatively impact aquaculture development in many fish species.
* The Asb15b gene is vital for skeletal muscle protein turnover and fiber development.

Purpose of the Study:

* To investigate the role of the Asb15b gene in intermuscular bone development using CRISPR/Cas9 technology.
* To assess the impact of Asb15b gene deletions on bone formation and muscle characteristics in zebrafish.

Main Methods:

* CRISPR/Cas9 gene editing to create Asb15b homozygous mutant zebrafish lines (-7 bp and -49 bp deletions).
* Skeleton staining, histology, and quantitative reverse transcription PCR (qRT-PCR) for analysis.

Main Results:

* Asb15b mutant zebrafish showed significant reductions in intermuscular bone number (30-40%).
* Muscle fiber diameter and number remained unchanged in mutants compared to wild-type.
* Key bone development regulators (bmp6, runx2b) showed altered expression in mutants.

Conclusions:

* The Asb15b gene is a critical regulator of intermuscular bone development in fish.
* Targeting Asb15b offers a potential strategy to reduce intermuscular bones in aquaculture species.