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Replication in Eukaryotes

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

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Related Experiment Video

Updated: Jun 26, 2025

Measuring Replicative Life Span in the Budding Yeast

Measuring Replicative Life Span in the Budding Yeast

Published on: June 25, 2009

Measuring Replicative Lifespan in Cryptococcus neoformans.

Vanessa K A Silva¹, Natalia K Oliveira², Bettina C Fries^3,4,5

¹Department of Medicine, Division of Infectious Diseases, Stony Brook University, Stony Brook, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)

|May 17, 2024

Summary

This summary is machine-generated.

Investigating yeast cell aging using microfluidic and microdissection tools helps understand Cryptococcus neoformans longevity. This research is crucial as aged yeast cells in infections exhibit increased virulence and antifungal resistance.

Keywords:

Aging Longevity Microdissection Microfluidic system Yeast

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Published on: June 29, 2018

Area of Science:

Microbiology and cellular biology
Yeast genetics and aging

Background:

Cellular aging research benefits from studying yeast replicative lifespan.
Understanding longevity in Cryptococcus neoformans is vital due to age-related phenotypes in infections.
Old yeast cells in infections correlate with higher virulence and antifungal tolerance.

Purpose of the Study:

To describe microdissection and microfluidic platforms for yeast cell aging studies.
To outline technical limitations and provide information for studying yeast aging mechanisms.
To assess the lifespan of yeast cells using advanced tracking methods.

Main Methods:

Utilizing microdissection for precise cell tracking.
Employing microfluidic devices for continuous single-cell analysis.
Comparing the features and limitations of both platforms for lifespan assessment.

Main Results:

Detailed description of microdissection and microfluidic device features.
Identification of technical challenges in single-cell yeast aging studies.
Framework for assessing yeast cell lifespan and aging mechanisms.

Conclusions:

Microdissection and microfluidic devices are powerful tools for yeast aging research.
Understanding yeast cell aging mechanisms is critical for pathogenic fungi like C. neoformans.
This chapter provides essential guidance for researchers studying cellular longevity and virulence.