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The human leucocyte P30 antigen: properties and assessment of value as a clonal marker
Abstract:
FMC3 and FMC29 are monoclonal antibodies which react with a 30,000 Dalton molecule expressed on the lymphocyte surface membrane. The molecule is a protein which does not appear to be N-glycosylated. The antigen, which is also expressed intracellularly, does not appear to be a marker of differentiation or maturation. Polyclonal cell populations, such as peripheral blood lymphocytes, show a bimodal distribution of surface antigen density, whilst monoclonal cell populations analysed quantitatively showed unimodal antigen density distributions. This suggests the antigen may be a clonal marker.
Insights
FMC3 and FMC29 monoclonal antibodies identify a 30,000 Dalton protein on lymphocytes. This lymphocyte surface antigen, not involved in differentiation, may serve as a clonal marker in monoclonal cell populations.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- FMC3 and FMC29 are monoclonal antibodies.
- These antibodies target a 30,000 Dalton molecule on the lymphocyte surface membrane.
Purpose of the Study:
- To characterize the 30,000 Dalton molecule recognized by FMC3 and FMC29.
- To investigate its potential role as a differentiation or clonal marker.
Main Methods:
- Monoclonal antibody characterization.
- Analysis of antigen expression on lymphocyte populations (polyclonal and monoclonal).
- Quantitative analysis of surface antigen density distribution.
Main Results:
- The target molecule is a non-N-glycosylated protein.
- The antigen is expressed both on the cell surface and intracellularly.
- It is not a marker of lymphocyte differentiation or maturation.
- Polyclonal populations show bimodal antigen density, while monoclonal populations show unimodal density.
Conclusions:
- The 30,000 Dalton antigen recognized by FMC3 and FMC29 is a cell surface protein.
- Its expression pattern suggests it may function as a clonal marker for lymphocytes.