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Published on: April 3, 2018
An in vitro assessment of teething gels' effects on human gingival mesenchymal stem cells
Sinem Birant1, Sabiha Ceren İlisulu2, Senem Kılıç3,4
1Faculty of Dentistry, Department of Pediatric Dentistry, Istanbul University-Cerrahpaşa, Istanbul, Turkey. sinembirant@iuc.edu.tr.
Background:
The aim of this study is to examine the cytotoxic effects of dental gels with different contents, which are frequently used during teething, on gingival mesenchymal stem cells (G-MSCs).
Method:
The teething gels used in this study were Dentinox, Gengigel, Osanite, and Jack and Jill. The human gingival mesenchimal stem cells (hG-MSCs) were incubated with these teething gel solutions (0.1%, 50% and 80% concentrations). Reproductive behavior of G-MSCs was monitored in real time for 72 h using the xCELLigence real-time cell analyzer (RTCA) system. Two-way repeated Anova test and post hoc Bonferroni test were used to evaluate the effect of concentration and dental gel on 0-hour and 72-hour viability. Significance was evaluated at p < 0.05 level.
Results:
Teething gels prepared at 50% concentration are added to the G-MSC culture, the "cell index" value of G-MSCs to which Dentinox brand gel is added is significantly lower than all other groups (p = 0.05). There is a statistically significant difference between the concentrations in terms of cell index values at the 72nd hour compared to the 0th hour (p = 0.001).
Conclusions:
The local anesthetic dental gels used in children have a more negative effect on cell viability as concentration increases.
