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Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats

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Timing of semen cryopreservation: before or after processing?

Ana Paula de Souza Kussler^1,2, Ivan Cunha Bustamante³, Elisa Negri¹

¹Universidade Federal do Rio Grande do Sul Faculdade de Medicina Porto AlegreRS Brazil Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.

Revista Brasileira De Ginecologia E Obstetricia : Revista Da Federacao Brasileira Das Sociedades De Ginecologia E Obstetricia

|May 20, 2024

Summary

Density gradient processing preserves sperm quality after cryopreservation, maintaining higher motility, viability, and morphology compared to other methods in normozoospermic patients. This technique enhances sperm parameters post-thawing.

Keywords:

Centrifugation Cryopreservation Density gradient Semen Seminal plasma Sperm count Sperm wash Spermatozoa

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Area of Science:

Reproductive Medicine
Sperm Biology
Cryobiology

Background:

Seminal cryopreservation can damage sperm, necessitating optimized processing methods.
Evaluating different techniques for seminal plasma removal is crucial for preserving sperm quality post-thaw.

Purpose of the Study:

To compare the efficacy of density gradient processing versus washing/centrifugation for seminal plasma removal prior to cryopreservation.
To assess the impact of these methods on key semen parameters after thawing.

Main Methods:

Semen samples from 26 normozoospermic patients were divided into three groups: with seminal plasma, after washing/centrifugation, and after density gradient processing.
All samples were cryopreserved for at least two weeks.
Sperm motility, count, morphology, and viability were evaluated before cryopreservation and after thawing.

Main Results:

Density gradient processing significantly improved the selection of motile, viable sperm with normal morphology in fresh samples (p<0.05).
Cryopreservation negatively impacted all semen parameters irrespective of the processing method.
Despite lower sperm recovery post-thaw, density gradient processing resulted in significantly higher progressive motility, total motility, viability, and morphology (p<0.05).

Conclusions:

Cryopreservation significantly compromises sperm motility, morphology, and viability.
Density gradient processing is superior in selecting higher-quality spermatozoa compared to other methods in normozoospermic individuals.
The benefits of density gradient processing for sperm quality are maintained even after cryopreservation and thawing.