Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phosphorylation01:02

Phosphorylation

50.3K
The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
50.3K
Replication in Eukaryotes02:31

Replication in Eukaryotes

170.6K
Overview
170.6K
Chromosome Replication02:31

Chromosome Replication

8.7K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
8.7K
The Replisome03:01

The Replisome

33.4K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
33.4K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.8K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.8K
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

5.5K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Telomere-Associated Proliferative Capacity in Expandable Porcine Hepatocyte-like Progenitor Cells.

Biology·2026
Same author

Recent neurodevelopmental outcomes of extremely and very preterm infants: A multicenter retrospective cohort study.

Early human development·2026
Same author

Feasibility of NIV-NAVA as a primary respiratory support and its clinical impacts in a targeted group of preterm infants: protocol for a prospective observational study.

BMJ open·2026
Same author

Protocol for cell cycle-resolved high-content imaging of transcription condensate dynamics in human cells.

STAR protocols·2026
Same author

ATR enforcement of the S/G2 checkpoint prevents premature S phase shutdown and genome instability.

bioRxiv : the preprint server for biology·2026
Same author

Polymerase face-off: emerging concepts in transcription-replication coordination.

EMBO reports·2026
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026
Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Jun 26, 2025

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

327

A phosphorylation code coordinating transcription condensate dynamics with DNA replication.

Carlos Origel Marmolejo, Celina Sanchez, Juyoung Lee

    Biorxiv : the Preprint Server for Biology
    |May 20, 2024
    PubMed
    Summary
    This summary is machine-generated.

    Nuclear condensates dynamically regulate gene transcription and DNA replication. Phosphorylation by cyclin-dependent kinases (CDKs) and ATR controls these processes, ensuring cell viability during proliferation.

    More Related Videos

    Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
    07:27

    Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

    Published on: April 29, 2010

    13.6K
    Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
    09:14

    Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

    Published on: January 14, 2016

    9.2K

    Related Experiment Videos

    Last Updated: Jun 26, 2025

    Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
    08:53

    Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

    Published on: May 2, 2025

    327
    Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
    07:27

    Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

    Published on: April 29, 2010

    13.6K
    Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
    09:14

    Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

    Published on: January 14, 2016

    9.2K

    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Biochemistry

    Background:

    • Chromatin organizes into functional compartments, including liquid condensates like transcription foci.
    • Mechanisms coordinating condensate dynamics with nuclear processes such as DNA replication remain largely unknown.

    Approach:

    • Investigated the G1/S cell cycle transition and the role of cyclin-dependent kinase 1 and 2 (CDK1/2) and ataxia-telangiectasia and Rad3-related (ATR) signaling.
    • Analyzed the phosphorylation of mediator subunit 1 (MED1) and its impact on condensate dynamics and DNA replication.

    Key Points:

    • Transcription condensates form at histone locus bodies (HLBs) dependently on CDK1/2 at G1/S transition.
    • ATR accumulates in HLBs and dissolves transcription condensates during S phase.
    • A phosphorylation code in MED1 integrates CDK1/2 and ATR signaling, coordinating condensate dynamics with DNA replication.

    Conclusions:

    • Spatiotemporal dynamics of transcription condensates are actively controlled by phosphorylation.
    • This regulation is essential for coordinating histone biosynthesis with DNA replication and maintaining genomic integrity in proliferating cells.