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Updated: Jun 26, 2025

Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy
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Accelerated protein retention expansion microscopy using microwave radiation.

Meghan R Bullard1, Juan Carlos Martinez Cervantes1, Norisha B Quaicoe1

  • 1Department of Biology, Georgetown University, Washington, DC 20057.

Biorxiv : the Preprint Server for Biology
|May 20, 2024
PubMed
Summary

Microwave-assisted expansion microscopy (ExM) significantly reduces protocol time from days to hours while maintaining high resolution. This accelerated method, microwave-assisted ExM (MW-ExM), is adaptable to various tissues and model organisms.

Keywords:
Drosophila melanogasterExpansion microscopyXenopus laevis tadpolesimmunohistochemistrymicrowaveneural tissue

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Area of Science:

  • Cell Biology
  • Microscopy Techniques
  • Neuroscience

Background:

  • Expansion microscopy (ExM) enables nanoscale imaging on conventional microscopes but is time-consuming.
  • Optimizing ExM protocols is crucial for broader biological applications.

Approach:

  • Adapted ExM for vibratome-sectioned Xenopus laevis tadpole brains using microwave assistance.
  • Developed microwave-assisted ExM (MW-ExM) to accelerate the protocol from days to hours.
  • Validated MW-ExM on whole mount adult Drosophila melanogaster brains, reducing a 6-day protocol to 2 days.

Key Points:

  • MW-ExM significantly reduces processing time while preserving high resolution and signal-to-noise ratio.
  • Achieved higher expansion magnitudes with MW-ExM compared to conventional ExM.
  • Demonstrated adaptability of MW-ExM to different model organisms (Xenopus, Drosophila) and tissue types.

Conclusions:

  • Microwave irradiation accelerates ExM by increasing reagent diffusion and potentially facilitating polymer expansion.
  • MW-ExM offers a highly efficient and adaptable method for nanoscale imaging in diverse biological studies.
  • Optimized microwave parameters allow customization for various specimens and ExM protocols.