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Updated: Jun 25, 2025

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
Published on: October 6, 2019
Switchable and orthogonal gene expression control inside artificial cells by synthetic riboswitches
Yuta Ishii1,2, Keisuke Fukunaga1, Aileen Cooney1,3
1Earth-Life Science Institute, Tokyo Institute of Technology, Ookayama 2-12-1, Meguro-Ku, Tokyo 152-8550, Japan. keisuke.fukunaga@elsi.jp.
Abstract:
Here we report two novel synthetic riboswitches that respond to ASP2905 and theophylline and function in reconstituted cell-free protein synthesis (CFPS) system. We encapsulated the CFPS system as well as DNA-templated encoding reporter genes regulated by these orthogonal riboswitches inside liposomes, and achieved switchable and orthogonal control over gene expression by external stimulation with the cognate ligands.
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