Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

4.0K
The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
 
Most enzymes...
4.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An Engineered Enzyme Catalyzing Tandem Reductive Aminations for Synthesizing Tertiary Amines.

Angewandte Chemie (International ed. in English)·2026
Same author

Chemoproteomics unveils the antibacterial mechanism of silver ions: inhibiting peptidoglycan synthesis <i>via</i> targeting Mur family proteins in <i>Staphylococcus aureus</i>.

Chemical science·2026
Same author

Towards the construction of a virtual yeast.

Nature·2026
Same author

Covalent Inhibition of SHMT2 by Gambogic Acid Induces Ferroptosis Through Mitochondrial Collapse in Triple-Negative Breast Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Programmable enhancement of endogenous mRNA translation through CRISPR-guided epitranscriptomic regulation.

Cell discovery·2026
Same author

Deep Learning-Guided Reverse Translation Enhances Soluble Expression of Recombinant Proteins in <i>Escherichia coli</i>.

International journal of molecular sciences·2026

Related Experiment Video

Updated: Jun 25, 2025

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

8.8K

REME: an integrated platform for reaction enzyme mining and evaluation.

Zhenkun Shi1, Dehang Wang1,2, Yang Li1,3

  • 1Biodesign Center, Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, PR China.

Nucleic Acids Research
|May 20, 2024
PubMed
Summary

Designing enzymes for new reactions is challenging. REME is a new web platform that helps researchers find and evaluate enzymes for non-natural reactions using advanced similarity searches and filtering tools.

More Related Videos

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

7.9K
GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization
11:38

GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

Published on: October 24, 2011

15.5K

Related Experiment Videos

Last Updated: Jun 25, 2025

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

8.8K
Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

7.9K
GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization
11:38

GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

Published on: October 24, 2011

15.5K

Area of Science:

  • Biocatalysis and enzyme engineering
  • Synthetic biology
  • Computational chemistry

Background:

  • Enzyme discovery for non-natural reactions presents significant challenges.
  • Existing computational tools for enzyme mining often lack accuracy and user interactivity.
  • Difficulty in evaluating and filtering numerous potential enzyme candidates hinders progress.

Purpose of the Study:

  • To introduce REME, the first integrated web platform for reaction enzyme mining and evaluation.
  • To provide researchers with an interactive tool for identifying and assessing enzymes for novel catalytic functions.
  • To overcome limitations of existing enzyme discovery platforms.

Main Methods:

  • Utilizing atom-to-atom mapping and reaction similarity calculations for ranking similar reactions.
  • Implementing interactive filtering based on functional groups and enzyme characteristics (e.g., organism, EC number).
  • Employing deep learning models to assess enzyme attributes like catalytic efficiency and optimal conditions.

Main Results:

  • REME enables rapid ranking and visualization of reactions similar to a target non-natural reaction.
  • The platform facilitates effective filtering and expansion of candidate enzymes.
  • Deep learning integration aids in swift identification of promising enzymes for non-natural catalysis.

Conclusions:

  • REME addresses key challenges in enzyme mining for pathway design.
  • The platform enhances the efficiency and accuracy of identifying biocatalysts for non-natural reactions.
  • REME empowers researchers to accelerate the development of novel enzymatic pathways.