Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Rab Proteins01:14

Rab Proteins

3.9K
Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
3.9K
Rab Cascades01:25

Rab Cascades

2.6K
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
2.6K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

2.5K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.5K
Intracellular Movement of Viruses and Bacteria01:10

Intracellular Movement of Viruses and Bacteria

2.8K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
2.8K
Types of RNA01:23

Types of RNA

63.6K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.6K
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

3.9K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
3.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Whole-genome sequence of <i>Bacillus anthracis</i> linked to the 2023 anthrax outbreak in Zambia.

Microbiology resource announcements·2026
Same author

<i>Legionella</i> employs the multimodal ubiquitination of Sec22b to modulate SNARE pairing.

iScience·2026
Same author

Phosphatidic acid production on the vacuole harboring <i>Legionella pneumophila</i> is a signal for recognition of interferon-induced GTPases.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Cleavage cascade of the sigma regulator FecR orchestrates TonB-dependent signal transduction.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Subversion of the host endocytic pathway by Legionella pneumophila-mediated ubiquitination of Rab5.

The Journal of cell biology·2025
Same author

Legionella uses host Rab GTPases and BAP31 to create a unique ER niche.

Cell reports·2024

Related Experiment Video

Updated: Jun 25, 2025

Tractable Mammalian Cell Infections with Protozoan-primed Bacteria
13:54

Tractable Mammalian Cell Infections with Protozoan-primed Bacteria

Published on: April 2, 2013

10.5K

Multi-tiered actions of Legionella effectors to modulate host Rab10 dynamics.

Tomoko Kubori1, Kohei Arasaki2, Hiromu Oide2

  • 1Department of Microbiology, Graduate School of Medicine, Gifu University, Gifu, Japan.

Elife
|May 21, 2024
PubMed
Summary

Legionella pneumophila manipulates Rab10 protein dynamics using ubiquitin signaling. Bacterial effectors control Rab10 recruitment and retention on the vacuole, fine-tuning host cell trafficking during infection.

Keywords:
Legionella pneumophilaRab10effectorinfectious diseasemicrobiologyubiquitin

More Related Videos

Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages
08:34

Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages

Published on: February 22, 2017

11.2K
Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
09:12

Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System

Published on: March 10, 2020

7.0K

Related Experiment Videos

Last Updated: Jun 25, 2025

Tractable Mammalian Cell Infections with Protozoan-primed Bacteria
13:54

Tractable Mammalian Cell Infections with Protozoan-primed Bacteria

Published on: April 2, 2013

10.5K
Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages
08:34

Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages

Published on: February 22, 2017

11.2K
Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
09:12

Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System

Published on: March 10, 2020

7.0K

Area of Science:

  • Cell Biology
  • Microbiology
  • Molecular Biology

Background:

  • Rab GTPases are key regulators of intracellular membrane trafficking.
  • Intracellular bacterial pathogens, like Legionella pneumophila, hijack host cell machinery, including Rab GTPases, for their survival and replication.
  • Understanding pathogen effector mechanisms is crucial for deciphering host-pathogen interactions.

Purpose of the Study:

  • To investigate the role of Rab GTPase regulation during Legionella pneumophila infection.
  • To elucidate the molecular mechanisms by which Legionella manipulates Rab10 dynamics.
  • To identify novel bacterial effectors involved in controlling Rab10.

Main Methods:

  • Utilized genetic manipulation of Legionella pneumophila strains.
  • Employed biochemical assays to study ubiquitination and effector activity.
  • Performed live-cell imaging to track Rab10 localization during infection.

Main Results:

  • Legionella pneumophila infection regulates Rab10 dynamics via ubiquitin signaling cascades mediated by SidE and SidC effector families.
  • Phosphoribosyl-ubiquitination by SidE ligases is essential for Rab10 recruitment to the bacterial vacuole.
  • The SidC effector SdcB retains Rab10 on the vacuole, while MavC suppresses SdcB function, leading to Rab10 removal.

Conclusions:

  • Legionella pneumophila employs a sophisticated interplay of bacterial effectors to precisely control Rab10 dynamics.
  • This fine-tuning of Rab10 localization is critical for the pathogen's manipulation of host membrane trafficking.
  • The study reveals novel mechanisms of host-pathogen interaction involving ubiquitin signaling and Rab GTPase regulation.