Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Fluid Mosaic Model01:34

The Fluid Mosaic Model

The fluid mosaic model was first proposed as a visual representation of research observations. The model comprises the composition and dynamics of membranes and serves as a foundation for future membrane-related studies. The model depicts the structure of the plasma membrane with a variety of components, which include phospholipids, proteins, and carbohydrates. These integral molecules are loosely bound, defining the cell’s border and providing fluidity for optimal function.
Typical Model Studies01:30

Typical Model Studies

Fluid mechanics model studies often utilize scaled-down systems to predict fluid behavior in full-scale environments, such as river flows, dam spillways, and structures interacting with open surfaces. Maintaining Froude number similarity in river models is crucial, as it replicates surface flow features like wave patterns and velocities.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multi-perspective analysis on the characteristics of composite non-point source pollution in typical hilly and plain urban aeras.

Journal of environmental sciences (China)·2026
Same author

The synthetic estradiol analog E0703 enhances <i>Akkermansia muciniphila</i> growth for radiation-induced intestinal damage repair.

mLife·2026
Same author

Self-derived Motion Features from sEMG for Inferring 3D Forearm Trajectories.

IEEE transactions on bio-medical engineering·2026
Same author

New insights into heterotrophic nitrification-aerobic denitrification during efficient pyridine degradation by Rhodococcus pyridinivorans WN2.

Bioresource technology·2026
Same author

Hemp seed oil mediates injury mitigation and anti-inflammation in radiated splenic T cells.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

High-altitude hypoxia drives dentate gyrus neuronal vulnerability through an IL1α-astrocyte-SLC1A2 pathway.

Journal of neuroinflammation·2026

Related Experiment Video

Updated: May 13, 2026

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
09:51

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

11.3K

A facile single-cell patterning strategy based on harbor-like microwell microfluidics.

Yingnan Sun1, Yongshu Liu1, Dezhi Sun2

  • 1Shandong Province Key Laboratory of Detection Technology for Tumor Makers, School of Medicine, Linyi University, Linyi 276005, People's Republic of China.

Biomedical Materials (Bristol, England)
|May 21, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces a novel microfluidic flip microwells chip for high-efficiency single-cell array preparation. This platform enables precise cell arrangement and analysis, overcoming limitations of existing methods for cell heterogeneity studies.

Keywords:
cellular heterogeneitymicrowell microfluidicssingle-cell array

More Related Videos

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly
10:17

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly

Published on: November 4, 2021

3.2K
Author Spotlight: Magnetic-Based Cell Patterning Method for High-Throughput Biomedical Applications
05:09

Author Spotlight: Magnetic-Based Cell Patterning Method for High-Throughput Biomedical Applications

Published on: February 2, 2024

1.4K

Related Experiment Videos

Last Updated: May 13, 2026

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
09:51

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

11.3K
Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly
10:17

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly

Published on: November 4, 2021

3.2K
Author Spotlight: Magnetic-Based Cell Patterning Method for High-Throughput Biomedical Applications
05:09

Author Spotlight: Magnetic-Based Cell Patterning Method for High-Throughput Biomedical Applications

Published on: February 2, 2024

1.4K

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • Single-cell analysis is crucial for understanding cellular heterogeneity, but current microfluidic microwell methods face limitations in cell size accommodation, capture efficiency, and spatiotemporal resolution.
  • Existing techniques often struggle with low single-cell capture rates and restrict reagent exchange and cell-to-cell communication.

Purpose of the Study:

  • To develop an improved microfluidic platform for high-efficiency single-cell array preparation and analysis.
  • To overcome the limitations of conventional microwell chips in handling diverse cell sizes and facilitating dynamic cellular studies.

Main Methods:

  • A novel microfluidic flip microwells chip platform was designed with large apertures (50 μm), shallow channels (50 μm), and deep microwells (50 μm).
  • Protein coating (bovine serum albumin and fibronectin) was employed to enhance cell adhesion.
  • The chip facilitates multi-cell trapping, single-cell array formation, and subsequent transfer to a planar surface for cell adherence and growth.

Main Results:

  • Achieved high single-cell capture efficiency of 92.1% ± 1% in microwells and 85% ± 3.4% on the planar microchannel.
  • Successfully demonstrated real-time, heterogeneous studies of single-cell calcium release and apoptosis behaviours.
  • The novel chip design integrates shallow channels, large apertures, and deep microwells for efficient cell acquisition without shearing forces.

Conclusions:

  • The microfluidic flip microwells chip platform offers a user-friendly and effective solution for high-efficiency single-cell array preparation.
  • This technology advances cell-based analysis and interdisciplinary research by enabling precise single-cell manipulation and observation.
  • The platform's design overcomes previous limitations, providing a valuable tool for various research fields.