Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 25, 2025

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice
05:59

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice

Published on: May 14, 2020

6.4K

Nylon Suture Loop for Tagging Lymphatic Channels during Lymphaticovenular Anastomosis.

Grace Hui-Min Tan1, Jiajun Feng1, Yee Onn Kok1

  • 1Plastic, Reconstructive and Aesthetic Surgery Service, Department of General Surgery, Sengkang General Hospital, Singapore, Singapore.

Indian Journal of Plastic Surgery : Official Publication of the Association of Plastic Surgeons of India
|May 22, 2024
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative Analysis of Electrocautery Versus LigaSure Energy-based Device for Anterolateral Thigh Free Flap Dissection.

Plastic and reconstructive surgery. Global open·2026
Same author

Adipofascial Flap Monitoring Using Masimo Regional Oximetry: A Case Report and Literature Review on Near-Infrared Spectroscopy for Flap Monitoring.

Archives of plastic surgery·2026
Same author

<i>Trichinella spiralis</i> HSP70 Mediates Mice Immune Responses via TLR2/MyD88/ERK Signaling Pathway.

Transboundary and emerging diseases·2026
Same author

Inhibition of starch retrogradation in fermented rice cakes during simulated cold chain logistics.

Food chemistry·2026
Same author

Medial Femoral Condyle Vascularized Graft for First Ray Arthrodesis and Reconstruction: A Case Report.

JBJS case connector·2026
Same author

Dynamic reorganization of three-dimensional genome architecture during Populus diversification.

Nature ecology & evolution·2026

A new supermicrosurgery technique uses a nylon suture and micro-ligaclip to tag lymphatic vessels during lymphaticovenular anastomosis (LVA). This method aids in precise lymphatic identification and improves surgical success rates.

Area of Science:

  • Vascular Surgery
  • Supermicrosurgery
  • Lymphatic System Research

Background:

  • Lymphaticovenular anastomosis (LVA) is a supermicrosurgical procedure for lymphatic disorders.
  • Handling delicate lymphatic vessels (<0.8 mm) during LVA preparation is challenging, risking injury or fracture.
  • Current methods lack optimal techniques for securing and identifying small lymphatic vessels.

Purpose of the Study:

  • To introduce a novel tagging technique for lymphatic vessels during LVA.
  • To enhance the ease and safety of lymphatic vessel manipulation in supermicrosurgery.
  • To improve the success rate of lymphaticovenular anastomosis procedures.

Main Methods:

  • A new technique involving a nylon 6-0 suture and a micro-ligaclip was developed for tagging lymphatic vessels.
Keywords:
lymphaticovenous anastomosislymphaticovenular anastomosismicro-ligaclipsupermicrosurgeryvessel loop

More Related Videos

Long-Term Catheterization of the Intestinal Lymph Trunk and Collection of Lymph in Neonatal Pigs
06:25

Long-Term Catheterization of the Intestinal Lymph Trunk and Collection of Lymph in Neonatal Pigs

Published on: March 5, 2016

9.5K
A Revised Method for Inducing Secondary Lymphedema in the Hindlimb of Mice
09:50

A Revised Method for Inducing Secondary Lymphedema in the Hindlimb of Mice

Published on: November 2, 2019

8.1K

Related Experiment Videos

Last Updated: Jun 25, 2025

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice
05:59

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice

Published on: May 14, 2020

6.4K
Long-Term Catheterization of the Intestinal Lymph Trunk and Collection of Lymph in Neonatal Pigs
06:25

Long-Term Catheterization of the Intestinal Lymph Trunk and Collection of Lymph in Neonatal Pigs

Published on: March 5, 2016

9.5K
A Revised Method for Inducing Secondary Lymphedema in the Hindlimb of Mice
09:50

A Revised Method for Inducing Secondary Lymphedema in the Hindlimb of Mice

Published on: November 2, 2019

8.1K
  • This "vessel loop" method facilitates secure handling and clear identification of target lymphatics.
  • The technique was applied in a series of LVA procedures.

    • Main Results:

    • The novel tagging technique was successfully employed in 78 lymphaticovenular anastomoses across 26 cases.
    • The average diameter of the lymphatic vessels treated was 0.3 mm, ranging from 0.15 mm to 0.8 mm.
    • The method proved effective in identifying and facilitating anastomosis with small lymphatic channels.

    Conclusions:

    • This nylon suture and micro-ligaclip tagging technique for lymphatic vessels is novel and previously undescribed.
    • The technique provides a significant benefit for lymphatic identification and handling in LVA.
    • It contributes to increased success rates in performing lymphaticovenular anastomosis.