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Related Concept Videos

RNA Splicing01:32

RNA Splicing

56.3K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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Real Time RT-PCR02:57

Real Time RT-PCR

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Related Experiment Video

Updated: Jun 25, 2025

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
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Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

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Timing is everything: advances in quantifying splicing kinetics.

Hope E Merens1, Karine Choquet2, Autum R Baxter-Koenigs1

  • 1Harvard University, Department of Genetics, Boston, MA, USA.

Trends in Cell Biology
|May 22, 2024
PubMed
Summary
This summary is machine-generated.

Splicing kinetics, the speed of RNA splicing, influences outcomes but was hard to study. New technologies now allow in vivo measurements, revealing factors that regulate splicing speed.

Keywords:
3′ splice site–RNA polymerase II distance-based measurementsco-transcriptional splicingsplicing kinetics

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Splicing is a crucial gene expression step, essential for cellular health.
  • Splicing is influenced by transcription, other splicing events, and regulatory factors.
  • Splicing kinetics, or the rate of splicing, can impact splicing outcomes but is difficult to measure in vivo.

Purpose of the Study:

  • To review technological advancements for measuring splicing kinetics in vivo.
  • To explore the variability of splicing kinetics at the single-intron level.
  • To understand how splicing kinetics are regulated within the cellular environment.

Main Methods:

  • Highlighting recent technological innovations for measuring global splicing kinetics.
  • Presenting methods for assessing splicing kinetic variability at single introns.
  • Analyzing features correlated with splicing kinetics.

Main Results:

  • New technologies enable direct measurement of splicing kinetics in vivo.
  • Variability in splicing kinetics can be observed at the single-intron level.
  • Correlations between features and splicing kinetics are being identified.

Conclusions:

  • Technological advancements have made studying splicing kinetics feasible.
  • Understanding splicing kinetics provides insights into gene regulation.
  • This research paves the way for developing models of in vivo splicing regulation.