Related Experiment Video
Updated: Jun 25, 2025

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
Published on: October 23, 2011
A detection system using sensing motif-tethered oligodeoxynucleotides for multiplex biomolecular analysis
Tatsuya Nishihara1, Yuto Motohashi1, Reoto Mio1
1Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University, 5-10-1 Fuchinobe, Chuo-ku, Sagamihara 252-5258, Japan. nishihara@chem.aoyama.ac.jp.
Abstract:
We developed a system to detect multiple target biomolecules through sensing motif-tethered oligodeoxynucleotides. DNA-based molecular probes gave the primary amine motif upon reaction with the target biomolecules, glutathione (GSH) and H2O2. After labelling with biotin, the product DNAs were selectively collected to be quantified by qPCR.

