Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis pathway,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

3D Printed Angiogenin-Functionalized Bioresorbable Tubular Conduits for Biological Vascularization.

ACS applied bio materials·2026
Same author

3D-printed skeletal tissue analogues using bone decellularized extracellular Matrix with PCL-bioglass composite: A biomimetic approach.

Colloids and surfaces. B, Biointerfaces·2026
Same author

Molecular detection of Mycobacterium tuberculosis complex (MTBC) in a wild Asian elephant (Elephas maximus) from Assam, India.

Veterinary research communications·2026
Same author

Low-level endemic circulation of porcine reproductive and respiratory syndrome virus in slaughtered pigs from Meghalaya, Northeast India: A cross-sectional seroepidemiological study.

Veterinary world·2026
Same author

ZW191, an FRα-Targeted Topoisomerase 1 Inhibitor ADC with a Differentiated Antitumor Efficacy and Tolerability Profile.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same author

Skeletal muscle tissue engineering using magnetic nanoparticles: a comprehensive review.

Journal of materials chemistry. B·2026

Related Experiment Video

Updated: Jun 26, 2026

Selection of Aptamers for Amyloid β-Protein, the Causative Agent of Alzheimer's Disease
15:23

Selection of Aptamers for Amyloid β-Protein, the Causative Agent of Alzheimer's Disease

Published on: May 13, 2010

19.4K

A Copper-Selective Sensor and Its Inhibition of Copper-Amyloid Beta Aggregation.

Ngoc Kim Nguyen1, Bella Poduska1, Mia Franks1

  • 1Carlson School of Chemistry and Biochemistry, Clark University, 950 Main Street, Worcester, MA 01610, USA.

Biosensors
|May 24, 2024
PubMed
Summary

Researchers developed a novel fluorescent probe to detect copper (Cu2+) and inhibit toxic amyloid-beta (Ab42) aggregation, offering potential therapeutic applications for Alzheimer's disease.

Keywords:
Cu-amyloid betafluorescent copper chelatornon-amyloid aggregate inhibition

More Related Videos

A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis
06:17

A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis

Published on: May 22, 2018

11.9K
Detecting Amyloid-β Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease
08:25

Detecting Amyloid-β Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease

Published on: April 19, 2021

3.3K

Related Experiment Videos

Last Updated: Jun 26, 2026

Selection of Aptamers for Amyloid β-Protein, the Causative Agent of Alzheimer's Disease
15:23

Selection of Aptamers for Amyloid β-Protein, the Causative Agent of Alzheimer's Disease

Published on: May 13, 2010

19.4K
A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis
06:17

A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis

Published on: May 22, 2018

11.9K
Detecting Amyloid-β Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease
08:25

Detecting Amyloid-β Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease

Published on: April 19, 2021

3.3K

Area of Science:

  • Biochemistry
  • Neuroscience
  • Chemical Biology

Background:

  • Copper is vital for biological processes but its dysregulation is linked to Alzheimer's disease.
  • Copper ions (Cu2+) aggregate with amyloid-beta (Ab42) peptides, contributing to senile plaque formation in Alzheimer's brains.
  • Selective detection and inhibition of Cu2+-Ab42 aggregates are crucial for therapeutic strategies.

Purpose of the Study:

  • To develop a Cu2+-selective fluorescent probe conjugated with an amyloid-beta binding peptide.
  • To create a hybrid molecule capable of detecting Cu2+-amyloid aggregates and reducing their size.
  • To investigate the potential therapeutic applications of this molecule in Alzheimer's disease.

Main Methods:

  • Synthesis of Cu2+-selective tri- and tetradentate ligands.
  • Conjugation of ligands with an amyloid-beta binding peptide.
  • Affinity and selectivity testing for Cu2+ and Cu2+-amyloid aggregates.
  • Fluorescence assays and microscopy to study aggregation inhibition.

Main Results:

  • Developed a highly selective Cu2+ chelator-peptide conjugate with strong fluorescence.
  • The probe detected Cu2+ at nanomolar concentrations and bound Cu2+ in a 1:1 ratio.
  • The conjugate efficiently reduced the size of Cu2+-Ab42 aggregates, inhibiting aggregation.

Conclusions:

  • The developed Cu2+-chelator-peptide conjugate shows high selectivity and fluorescence for Cu2+ detection.
  • This hybrid molecule effectively inhibits Cu2+-Ab42 aggregation, reducing aggregate size.
  • The compound holds significant potential as a therapeutic agent for Alzheimer's disease.