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Related Experiment Video

Updated: Jun 25, 2025

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Published on: August 7, 2021

Accessible high-speed image-activated cell sorting.

Terra M Kuhn¹, Malte Paulsen², Sara Cuylen-Haering¹

¹Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.

Trends in Cell Biology

|May 24, 2024

Summary

This summary is machine-generated.

Recent advancements in flow cytometry integrate microscopy and cell sorting, enabling high-throughput isolation of cells based on image analysis. This technology expands applications in life science research and clinical settings.

Keywords:

cell sorting flow cytometry high-throughput imaging image analysis

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Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

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Last Updated: Jun 25, 2025

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Published on: August 7, 2021

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting FACS

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting FACS

Published on: July 10, 2010

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Published on: November 4, 2016

Area of Science:

Biotechnology
Cell Biology
Medical Technology

Background:

Fluorescence-activated cell sorting (FACS) has been a cornerstone of biological research for over 60 years, facilitating high-throughput cell isolation.
Traditional FACS relies on fluorescent markers and fluid dynamics for cell separation.

Purpose of the Study:

To review emerging flow cytometry systems that combine microscopy with high-speed cell sorting.
To discuss the capabilities, limitations, and potential applications of these advanced imaging-based cell sorting technologies.

Main Methods:

Review of commercially available and replicable imaging-enhanced flow cytometry systems.
Analysis of system strengths, limitations, and engineering details.
Summarization of current and potential applications in research and clinical settings.

Main Results:

New instruments integrate spatial resolution of microscopy with high-speed cell sorting.
Cell sorting can be based on image-derived parameters and sophisticated image analysis algorithms.
These advancements significantly broaden the scope of applications for cell sorting.

Conclusions:

Imaging-enhanced flow cytometry represents a new era in cell analysis and isolation.
These technologies hold transformative potential for basic life science and clinical applications.
Further development and adoption of these systems are expected to drive innovation in biological research.