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High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis

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Choosing a camera and optimizing system parameters for speckle contrast optical spectroscopy.

Tom Y Cheng^1,2,3, Byungchan Kim¹, Bernhard B Zimmermann¹

¹Department of Biomedical Engineering, Neurophotonics Center, Boston University, Boston, MA, 02215, USA.

Scientific Reports

|May 24, 2024

Summary

Selecting the right camera is crucial for accurate speckle contrast optical spectroscopy (SCOS) measurements of cerebral blood flow (CBF). This study guides camera selection and optimization for improved SCOS performance.

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Area of Science:

Biomedical Optics
Neuroimaging
Optical Instrumentation

Background:

Speckle contrast optical spectroscopy (SCOS) is a camera-based method for measuring cerebral blood flow (CBF).
Camera noise and nonidealities at low photon flux can degrade SCOS accuracy in human CBF monitoring.
Optimizing camera selection is vital for advancing optical devices for brain function monitoring.

Purpose of the Study:

To provide a comprehensive guide for characterizing, selecting, and optimizing cameras for SCOS measurements.
To evaluate the suitability of commercially available cameras for accurate SCOS-based CBF quantification.
To establish optimal operating parameters for SCOS systems using low-cost cameras.

Main Methods:

Evaluation of three complementary metal-oxide-semiconductor (CMOS) cameras based on linearity, read noise, and quantization distortion.
Assessment of camera performance for spatial contrast quantification in SCOS.
Determination of optimal operating parameters for the selected camera.

Main Results:

Not all cameras suitable for general imaging perform well in SCOS due to challenges in quantifying spatial contrast.
A preferred low-cost camera was identified and its optimal operating parameters were determined.
Successful measurement of human CBF was demonstrated using the selected camera and optimized parameters.

Conclusions:

A systematic guideline for camera selection and parameter optimization in SCOS is established.
This work facilitates the development of next-generation optical devices for monitoring human CBF and brain function.
The findings enable accurate and reliable SCOS measurements using optimized, cost-effective camera technology.