Cross Talking Calcium, IP₃ and Buffer Dynamics Alters ATP and NADH Level in Obese and Normal Hepatocyte Cell

The cross talk between calcium (Ca²⁺), IP₃ and buffer dynamics regulate various mechanisms in hepatocyte cells. The study of independent systems of calcium, IP₃, and buffer signaling provides limited information about cell dynamics. In the current study, coupled reaction-diffusion equations are used to design a cross-talk model for IP₃, buffer, and calcium dynamics in a hepatocyte cell. The one-way feedback of calcium, buffer, and IP₃ in ATP production, ATP degradation, and NADH production rate is incorporated into the model. Numerical simulation has been done using the Finite Element Method (FEM) along the spatial direction and the Crank-Nicolson (C-N) method along the temporal direction. The numerical results are analysed to determine the effects of alterations in processes of cross-talking dynamics of IP₃, buffer, and calcium on ATP and NADH production and degradation rate of ATP in a hepatocyte cell under normal and obesity conditions. The comparative analysis of these findings unveils notable distinctions induced by obesity in calcium dynamics, ATP and NADH synthesis, and ATP degradation kinetics.