Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical activity and safety of nivolumab in combination with ipilimumab in metastatic melanoma: findings from REALIPINIVO, a real-world study.

Frontiers in immunology·2026
Same author

Effects of Vericiguat on the Mitochondrial Function and Clinical Outcomes in CRT Nonresponders Patients.

JACC. Advances·2026
Same author

Neuronal VIP shapes intestinal stem cell activity and mucosal immunity.

Cell stem cell·2026
Same author

In vitro evidence and integrative bioinformatics identify the SGLT2-PPARγ axis as a target against polyethylene microplastic-driven metabolic reprogramming in colorectal cancer cells.

Journal of translational medicine·2026
Same author

Metastatic Syringocystadenocarcinoma Papilliferum Treated with Enzalutamide: A Case Report and a Review of the Literature.

Case reports in dermatology·2026
Same author

PCSK9 inhibition ameliorates microplastic-induced endothelial redox imbalance via SIRT6 modulation.

Cellular & molecular biology letters·2025

Related Experiment Video

Updated: Jun 25, 2025

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
12:50

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Published on: September 15, 2017

6.6K

MiR-148a-3p/SIRT7 Axis Relieves Inflammatory-Induced Endothelial Dysfunction.

Camilla Anastasio1, Isabella Donisi1, Antonino Colloca1

  • 1Department of Precision Medicine, University of Campania Luigi Vanvitelli, Via L. De Crecchio 7, 80138 Naples, Italy.

International Journal of Molecular Sciences
|May 25, 2024
PubMed
Summary

MicroRNA-148a-3p protects endothelial cells from inflammation-induced mitochondrial dysfunction and apoptosis by targeting SIRT7. This finding reveals a new therapeutic target for preventing endothelial dysfunction.

Keywords:
SIRT7apoptosisendothelial dysfunctioninflammationmiR-148a-3pmitochondrial damageoxidative stress

More Related Videos

On-Chip Endothelial Inflammatory Phenotyping
12:43

On-Chip Endothelial Inflammatory Phenotyping

Published on: July 21, 2012

10.9K
Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly
00:06

Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly

Published on: August 20, 2019

15.2K

Related Experiment Videos

Last Updated: Jun 25, 2025

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
12:50

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Published on: September 15, 2017

6.6K
On-Chip Endothelial Inflammatory Phenotyping
12:43

On-Chip Endothelial Inflammatory Phenotyping

Published on: July 21, 2012

10.9K
Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly
00:06

Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly

Published on: August 20, 2019

15.2K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • MicroRNA-148a-3p (miR-148a-3p) plays a role in endothelial cell pathology, but its precise mechanism in inflammatory conditions remains unclear.
  • Interleukin-6 (IL-6) is a key mediator of vascular dysfunction and inflammation.

Purpose of the Study:

  • To investigate the role of miR-148a-3p in mitochondrial dysfunction and apoptosis in human aortic endothelial cells (teloHAECs) under IL-6 stimulation.
  • To elucidate the molecular mechanism linking miR-148a-3p, SIRT7, and IL-6-induced endothelial inflammation.

Main Methods:

  • Human aortic endothelial cells (teloHAECs) were treated with IL-6.
  • miR-148a-3p expression, mitochondrial stress, and apoptosis were assessed.
  • miR-148a-3p mimic was used to evaluate its protective effects.
  • Bioinformatics analysis and dual-luciferase reporter assays were employed to validate the interaction between miR-148a-3p and SIRT7.

Main Results:

  • IL-6 treatment decreased miR-148a-3p expression and increased SIRT7 levels, mitochondrial stress, and apoptosis in teloHAECs.
  • Restoring miR-148a-3p levels using a mimic counteracted IL-6 effects, reducing cytokine release, apoptosis, and improving mitochondrial function.
  • miR-148a-3p directly targets SIRT7 mRNA, downregulating its expression.

Conclusions:

  • The miR-148a-3p/SIRT7 axis plays a critical role in mitigating mitochondrial damage and apoptosis in endothelial cells during inflammation.
  • This pathway represents a novel therapeutic target for preventing IL-6-mediated endothelial dysfunction.