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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Correction: Kang et al. Fluid Flow to Electricity: Capturing Flow-Induced Vibrations with Micro-Electromechanical-System-Based Piezoelectric Energy Harvester. <i>Micromachines</i> 2024, <i>15</i>, 581.

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Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
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A Silicon-Based ROTE Sensor for High-Q and Label-Free Carcinoembryonic Antigen Detection.

Luxiao Sang1,2, Haojie Liang1,2,3, Biao Zhao1,2

  • 1Shanxi Key Laboratory of Micro-Nano Sensors & Artificial Intelligence Perception, College of Electronic Information and Optical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.

Micromachines
|May 25, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces a novel biosensor using resonant optical tunneling effect (ROTE) to detect carcinoembryonic antigen (CEA). The biosensor shows promise for early cancer detection by accurately measuring CEA concentrations.

Keywords:
carcinoembryonic antigen detectionoptical biosensorquality factorresonant optical tunneling effect

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Area of Science:

  • Biomedical Engineering
  • Optoelectronics
  • Biosensing Technology

Background:

  • Carcinoembryonic antigen (CEA) is a crucial biomarker for early cancer detection.
  • Existing biosensing methods require sensitive and specific detection techniques.
  • Optical biosensors offer label-free detection with high sensitivity.

Purpose of the Study:

  • To develop and characterize a novel biosensor for detecting CEA.
  • To utilize the resonant optical tunneling effect (ROTE) for enhanced sensitivity.
  • To establish a method for early cancer biomarker identification.

Main Methods:

  • Fabrication of a biosensor based on resonant optical tunneling effect (ROTE).
  • Immobilization of anti-CEA antibodies on the sensor surface for CEA capture.
  • Detection of CEA through changes in refractive index and wavelength shifts.
  • Analysis of wavelength shifts via cascade coupling of FP and ROTE cavities.

Main Results:

  • The biosensor demonstrated a linear response to CEA concentrations from 1 to 5 ng/mL.
  • A limit of detection (LOD) of 0.5 ng/mL was achieved.
  • The biosensor exhibited a high total Q factor of 9500.
  • Resonance wavelength shifts correlated directly with increasing CEA concentration.

Conclusions:

  • The ROTE-based biosensor is a sensitive and effective tool for CEA detection.
  • This technology offers a new pathway for early cancer biomarker identification.
  • The biosensor's performance highlights its potential for clinical diagnostics.