Related Experiment Video
Updated: May 12, 2026
07:40
Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications
Published on: June 29, 2020
13.4K
Rapid Detection and Quick Characterization of African Swine Fever Virus Using the VolTRAX Automated Library
Vivian O'Donnell1, Jim L Pierce2, Oleg Osipenko3
1Plum Island Animal Disease Center, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Foreign Animal Disease Diagnostic Laboratory, National Veterinary Services Laboratories, Orient, NY 11957, USA.
Viruses
|May 25, 2024
Summary
Automated DNA library preparation using Oxford Nanopore
Area of Science:
- Veterinary Virology
- Genomic Sequencing
- Molecular Diagnostics
Background:
- African swine fever virus (ASFV) causes a highly contagious and fatal disease in swine.
- ASFV outbreaks severely impact global pig production and international trade.
- Rapid ASFV detection is crucial for disease containment.
Purpose of the Study:
- To evaluate the Oxford Nanopore Technologies' VolTRAX platform for automated DNA library preparation.
- To assess the feasibility of rapid ASFV strain identification using VolTRAX and Nanopore sequencing.
Main Methods:
- Automated DNA library preparation for ASFV using the VolTRAX platform.
- Downstream sequencing on Nanopore sequencing platforms (MinION Mk1C and GridION).
- Analysis of generated near-full genome sequences for ASFV identification.
Main Results:
- VolTRAX enabled rapid DNA library preparation for ASFV within minutes.
- Near-full genome sequences of ASFV were obtained rapidly.
- The automated workflow demonstrated effectiveness for ASFV detection.
Conclusions:
- The VolTRAX platform is an effective tool for automated library preparation.
- This automated method facilitates rapid and real-time detection of ASFV.
- The workflow is suitable for both field (MinION) and laboratory (GridION) settings.
