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Published on: February 20, 2020
SPECT Imaging of P. aeruginosa Infection in Mice Using 123I-BMIPP
Yuri Nishiyama1,2, Asuka Mizutani3, Masato Kobayashi3
1Division of Health Sciences, Graduate School of Medical Sciences, Kanazawa University, 5-11-80 Kodatsuno, Kanazawa 920-0942, Japan.
Abstract:
Pseudomonas aeruginosa infection is an infectious disease that must be controlled because it becomes chronic and difficult to treat, owing to its unique system of toxin production/injection and elimination of other bacteria. Here, we noninvasively monitored P. aeruginosa using single-photon emission computed tomography (SPECT) imaging. Determining the amount and localization of the P. aeruginosa will enable making faster clinical diagnoses and selecting the most appropriate therapeutic agents and methods. Nonclinically, this information can be used for imaging in combination with biofilms and toxin probes and will be useful for discovering drugs targeting P. aeruginosa. To study P. aeruginosa accumulation, we conducted in vitro and in vivo studies using iodine-123 β-methyl-p-iodophenyl-pentadecanoic acid (123I-BMIPP), which we previously reported using for Escherichia coli. In vitro, 123I-BMIPP accumulated in P. aeruginosa by being taken up into the bacteria and adsorbing to the bacterial surface. In vivo, 123I-BMIPP accumulated significantly more in infected sites than in noninfected sites and could be quantified by SPECT. These results suggest that 123I-BMIPP can be used as a probe for P. aeruginosa for SPECT. Establishing a noninvasive monitoring method using SPECT will allow further progress in studying P. aeruginosa.
Insights
Noninvasive imaging of Pseudomonas aeruginosa infections using SPECT and 123I-BMIPP allows for better diagnosis and treatment selection. This method aids in understanding bacterial accumulation for drug discovery and research.
Area of Science:
- Medical Imaging
- Infectious Diseases
- Bacteriology
Background:
- Pseudomonas aeruginosa infections are challenging due to chronicity and resistance.
- Effective control requires understanding bacterial load and location.
- Current diagnostic methods may be invasive or lack precision.
Purpose of the Study:
- To develop a noninvasive method for monitoring Pseudomonas aeruginosa using SPECT imaging.
- To evaluate the efficacy of iodine-123 β-methyl-p-iodophenyl-pentadecanoic acid (123I-BMIPP) as a SPECT imaging probe for P. aeruginosa.
- To determine the accumulation and localization of P. aeruginosa in vitro and in vivo.
Main Methods:
- Utilized single-photon emission computed tomography (SPECT) imaging.
- Employed 123I-BMIPP, a previously reported imaging agent, for P. aeruginosa studies.
- Conducted in vitro bacterial uptake and adsorption experiments.
- Performed in vivo SPECT imaging in infected and noninfected sites.
Main Results:
- 123I-BMIPP demonstrated accumulation in P. aeruginosa through bacterial uptake and surface adsorption in vitro.
- SPECT imaging revealed significantly higher 123I-BMIPP accumulation at infected sites compared to noninfected sites in vivo.
- The accumulation of 123I-BMIPP at infection sites was quantifiable using SPECT.
Conclusions:
- 123I-BMIPP shows potential as a SPECT imaging probe for noninvasive monitoring of Pseudomonas aeruginosa infections.
- This SPECT-based approach facilitates faster clinical diagnosis and targeted therapeutic strategies.
- The method supports further research into P. aeruginosa biofilms, toxin activity, and drug discovery.
